Simizu B, Abe S, Yamamoto H, Tano Y, Ota Y, Miyazawa M, Horie H, Satoh K, Wakabayashi K
Japan Poliomyelitis Research Institute, 5-34-4, Kumegawa-cho, Higashi-murayama, Tokyo 189-0003, Japan.
Biologicals. 2006 Jun;34(2):151-4. doi: 10.1016/j.biologicals.2006.02.010. Epub 2006 May 5.
In the course of Sabin-inactivated poliovirus vaccine (S-IPV) development, we have established high-yield virus production techniques based on Vero cell micro-carrier cultures. Development of specific ELISA tests to quantify the antigen content of S-IPV has been achieved. To adjust the immunogenicity of S-IPV so as to be comparable with the conventional-IPV, a new formulation was determined using a potency test using rats. The reformulated S-IPV was shown to be efficacious for the immunization of monkeys.
在沙宾灭活脊髓灰质炎病毒疫苗（S-IPV）的研发过程中，我们基于Vero细胞微载体培养建立了高产病毒生产技术。已开发出特异性ELISA检测方法来定量S-IPV的抗原含量。为了调整S-IPV的免疫原性使其与传统IPV相当，通过大鼠效力试验确定了一种新配方。重新配方的S-IPV对猴子免疫显示出有效性。
