Kersten G, Hazendonk T, Beuvery C
Institute of Public Health and the Environment, Laboratory for Product and Process Development, Bilthoven, The Netherlands.
Vaccine. 1999 Apr 9;17(15-16):2059-66. doi: 10.1016/s0264-410x(98)00409-5.
Inactivated polio vaccine (IPV) was prepared with the Sabin strains, normally used for the attenuated live vaccine. The vaccine was characterized with respect to its antigenicity as determined by ELISA and biosensor analysis and its immunogenicity in rats. Compared with the vaccine prepared with virulent strains (Mahoney, MEF and Saukett) some distinct differences were found with regard to the interaction with glass vials (types 1 and 2) and antigenicity (type 3). The most profound difference however, was the immunogenicity of types 1 and 2. Standardized on the amount of virus, type 1 Sabin-IPV was about 3 times more immunogenic as compared to Mahoney-IPV. The immunogenicity of type 2 Sabin-IPV on the other hand was reduced approximately 10-fold, compared to MEF-IPV. Type 3 Sabin and Saukett IPV were comparable in immunogenicity but differences in antigenicity were evident. The lack of correlation between antigenicity and immunogenicity demonstrate that current IPV standards are not suitable to quantify IPV made from Sabin strains. The results indicate that future Sabin-IPV vaccines may have to contain virus amounts that are very different as compared to current IPV vaccines.
灭活脊髓灰质炎疫苗（IPV）是用通常用于减毒活疫苗的萨宾毒株制备的。通过ELISA和生物传感器分析确定了该疫苗的抗原性，并在大鼠中检测了其免疫原性。与用强毒株（马奥尼、MEF和索基特）制备的疫苗相比，发现其在与1型和2型玻璃瓶的相互作用以及抗原性（3型）方面存在一些明显差异。然而，最显著的差异在于1型和2型的免疫原性。以病毒量为标准，1型萨宾IPV的免疫原性比马奥尼IPV高约3倍。另一方面，与MEF-IPV相比，2型萨宾IPV的免疫原性降低了约10倍。3型萨宾和索基特IPV在免疫原性方面相当，但抗原性存在明显差异。抗原性和免疫原性之间缺乏相关性表明，目前的IPV标准不适用于量化由萨宾毒株制成的IPV。结果表明，未来的萨宾IPV疫苗可能必须含有与目前IPV疫苗非常不同的病毒量。
