Cruzeiro-Silva C, Albernaz F P, Valente A P, Almeida F C L
Centro Nacional de Ressonância Magnética Nuclear Jiri Jonas-CNRMN, Instituto de Bioquímica Médica, Programa de Biologia Estrutural, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, Brazil.
Cell Biochem Biophys. 2006;44(3):497-502. doi: 10.1385/CBB:44:3:497.
Structural studies by in-cell nuclear magnetic resonance are a developing new field of research, and their objective is to obtain structural information of proteins and other biological macromolecules in the cytoplasm of Escherichia coli cells. The major limitation of in-cell experiments is cell lysis that occurs during the experiments. In this article, we describe how inhibition of autologous expression by rifampicin at a high concentration decreases cell lysis in E. coli. We suggest that rifampicin is acting in the programmed cell death gene system MazEF, which is triggered by stress conditions and ultimately leads to cell lysis.
通过细胞内核磁共振进行的结构研究是一个正在发展的新研究领域，其目的是获取大肠杆菌细胞质中蛋白质和其他生物大分子的结构信息。细胞内实验的主要局限性在于实验过程中发生的细胞裂解。在本文中，我们描述了高浓度利福平对自体表达的抑制如何减少大肠杆菌中的细胞裂解。我们认为利福平作用于程序性细胞死亡基因系统MazEF，该系统由应激条件触发并最终导致细胞裂解。
