细胞内 NMR 光谱法研究天然无序蛋白质
In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.
机构信息
Department of Chemistry, University at Albany, State University of New York, Albany, NY, 12222, USA.
出版信息
Proteomics. 2019 Mar;19(6):e1800055. doi: 10.1002/pmic.201800055. Epub 2019 Jan 15.
Abstract
This review summarizes the results of in-cell Nuclear Magnetic Resonance, NMR, spectroscopic investigations of the eukaryotic and prokaryotic intrinsically disordered proteins, IDPs: α-synuclein, prokaryotic ubiquitin-like protein, Pup, tubulin-related neuronal protein, Tau, phenylalanyl-glycyl-repeat-rich nucleoporins, FG Nups, and the negative regulator of flagellin synthesis, FlgM. The results show that the cellular behavior of IDPs may differ significantly from that observed in the test tube.
摘要
本综述总结了真核生物和原核生物无规卷曲蛋白（IDP）：α-突触核蛋白、原核泛素样蛋白 Pup、微管相关神经元蛋白 Tau、苯丙氨酸-甘氨酸重复丰富核孔蛋白 FG Nups 和鞭毛合成负调节剂 FlgM 的细胞内核磁共振（NMR）光谱研究结果。结果表明，IDP 的细胞行为可能与在试管中观察到的行为有很大的不同。
相似文献
1
In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.细胞内 NMR 光谱法研究天然无序蛋白质
Proteomics. 2019 Mar;19(6):e1800055. doi: 10.1002/pmic.201800055. Epub 2019 Jan 15.
2
Determining Binding Kinetics of Intrinsically Disordered Proteins by NMR Spectroscopy.通过 NMR 光谱法测定天然无序蛋白质的结合动力学。
Methods Mol Biol. 2020;2141:663-681. doi: 10.1007/978-1-0716-0524-0_34.
3
Interactions of IDPs with Membranes Using Dark-State Exchange NMR Spectroscopy.用暗态交换 NMR 光谱法研究 IDPs 与膜的相互作用。
Methods Mol Biol. 2020;2141:585-608. doi: 10.1007/978-1-0716-0524-0_30.
4
Characterization of Dynamic IDP Complexes by NMR Spectroscopy.通过核磁共振光谱对动态内在无序蛋白复合物进行表征
Methods Enzymol. 2018;611:193-226. doi: 10.1016/bs.mie.2018.08.026. Epub 2018 Oct 29.
5
Characterization of intrinsically disordered proteins and their dynamic complexes: From in vitro to cell-like environments.无定形蛋白质及其动态复合物的特性：从体外到类细胞环境。
Prog Nucl Magn Reson Spectrosc. 2018 Dec;109:79-100. doi: 10.1016/j.pnmrs.2018.07.001. Epub 2018 Jul 31.
6
Ensemble characterization of an intrinsically disordered FG-Nup peptide and its F>A mutant in DMSO-d.在氘代二甲亚砜中对一种内在无序的FG核孔蛋白肽及其F>A突变体进行整体表征。
Biopolymers. 2017 Nov;108(6). doi: 10.1002/bip.23036. Epub 2017 Jul 22.
7
C APSY-NMR for sequential assignment of intrinsically disordered proteins.用于内在无序蛋白质序列归属的CAPSY-NMR
J Biomol NMR. 2018 Mar;70(3):167-175. doi: 10.1007/s10858-018-0167-4. Epub 2018 Feb 28.
8
High-dimensional NMR methods for intrinsically disordered proteins studies.用于研究无规卷曲蛋白质的高维 NMR 方法。
Methods. 2018 Sep 15;148:81-87. doi: 10.1016/j.ymeth.2018.04.031. Epub 2018 Apr 27.
9
NMR Spectroscopic Studies of the Conformational Ensembles of Intrinsically Disordered Proteins.内在无序蛋白质构象集合的核磁共振光谱研究。
Adv Exp Med Biol. 2015;870:149-85. doi: 10.1007/978-3-319-20164-1_5.
10
NMR contributions to structural dynamics studies of intrinsically disordered proteins.核磁共振对内在无序蛋白质结构动力学研究的贡献。
J Magn Reson. 2014 Apr;241(100):74-85. doi: 10.1016/j.jmr.2013.11.011.
引用本文的文献
1
Exploring Intrinsic Disorder in Human Synucleins and Associated Proteins.探索人类突触核蛋白和相关蛋白中的内源性无序。
Int J Mol Sci. 2024 Aug 1;25(15):8399. doi: 10.3390/ijms25158399.
2
3
Similarity of the non-amyloid-β component and C-terminal tail of monomeric and tetrameric alpha-synuclein with 14-3-3 sigma.单体和四聚体α-突触核蛋白的非淀粉样β成分及C末端尾巴与14-3-3西格玛的相似性。
Comput Struct Biotechnol J. 2021 Sep 14;19:5348-5359. doi: 10.1016/j.csbj.2021.09.011. eCollection 2021.
4
F NMR as a tool in chemical biology.F核磁共振作为化学生物学中的一种工具。
Beilstein J Org Chem. 2021 Jan 28;17:293-318. doi: 10.3762/bjoc.17.28. eCollection 2021.
5
Liquid-Liquid Phase Separation by Intrinsically Disordered Protein Regions of Viruses: Roles in Viral Life Cycle and Control of Virus-Host Interactions.病毒无规则蛋白区域的液-液相分离：在病毒生命周期中的作用和对病毒-宿主相互作用的控制。
Int J Mol Sci. 2020 Nov 28;21(23):9045. doi: 10.3390/ijms21239045.
6
Perspective: the essential role of NMR in the discovery and characterization of intrinsically disordered proteins.观点：NMR 在无规卷曲蛋白质的发现和特性描述中的重要作用。
J Biomol NMR. 2019 Dec;73(12):651-659. doi: 10.1007/s10858-019-00280-2. Epub 2019 Oct 15.
7
In Vivo Methods to Study Protein-Protein Interactions as Key Players in Virulence.研究蛋白质-蛋白质相互作用作为毒力关键因素的体内方法
Pathogens. 2019 Oct 1;8(4):173. doi: 10.3390/pathogens8040173.
8
Living in Promiscuity: The Multiple Partners of Alpha-Synuclein at the Synapse in Physiology and Pathology.在突触中生活的杂乱无章：α-突触核蛋白的多重伴侣在生理和病理中的作用。
Int J Mol Sci. 2019 Jan 2;20(1):141. doi: 10.3390/ijms20010141.
9
In-Cell NMR Study of Tau and MARK2 Phosphorylated Tau.细胞内 NMR 研究 Tau 和 MARK2 磷酸化 Tau。
Int J Mol Sci. 2018 Dec 26;20(1):90. doi: 10.3390/ijms20010090.
本文引用的文献
1
Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening.利用基于细胞内 NMR 的筛选技术鉴定出结核分枝杆菌生长的有效抑制剂。
ACS Chem Biol. 2018 Mar 16;13(3):733-741. doi: 10.1021/acschembio.7b00879. Epub 2018 Feb 2.
2
Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions.实时细胞内核磁共振：核糖体靶向抗生素调节五元蛋白质相互作用。
Biochemistry. 2018 Feb 6;57(5):540-546. doi: 10.1021/acs.biochem.7b00938. Epub 2018 Jan 8.
3
Impact of cellular health conditions on the protein folding state in mammalian cells.细胞健康状况对哺乳动物细胞中蛋白质折叠状态的影响。
Chem Commun (Camb). 2017 Oct 10;53(81):11245-11248. doi: 10.1039/c7cc06004a.
4
Ribosome Mediated Quinary Interactions Modulate In-Cell Protein Activities.核糖体介导的五元相互作用调节细胞内蛋白质活性。
Biochemistry. 2017 Aug 15;56(32):4117-4126. doi: 10.1021/acs.biochem.7b00613. Epub 2017 Aug 3.
5
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.利用顺磁核磁共振光谱法测定活细胞中蛋白质的三维结构
Chem Commun (Camb). 2016 Aug 11;52(67):10237-40. doi: 10.1039/c6cc05490k.
6
In-Cell Protein Structures from 2D NMR Experiments.通过二维核磁共振实验获得的细胞内蛋白质结构
J Phys Chem Lett. 2016 Jul 21;7(14):2821-5. doi: 10.1021/acs.jpclett.6b01074. Epub 2016 Jul 12.
7
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.在培养的哺乳动物细胞中通过细胞内 NMR 光谱学对蛋白质进行表征。
Nat Protoc. 2016 Jun;11(6):1101-11. doi: 10.1038/nprot.2016.061. Epub 2016 May 19.
8
Small molecules, big targets: drug discovery faces the protein-protein interaction challenge.小分子，大目标：药物发现面临蛋白-蛋白相互作用挑战。
Nat Rev Drug Discov. 2016 Aug;15(8):533-50. doi: 10.1038/nrd.2016.29. Epub 2016 Apr 11.
9
Structural disorder of monomeric α-synuclein persists in mammalian cells.单体α-突触核蛋白的结构无序在哺乳动物细胞中持续存在。
Nature. 2016 Feb 4;530(7588):45-50. doi: 10.1038/nature16531. Epub 2016 Jan 25.
10
The Synaptic Function of α-Synuclein.α-突触核蛋白的突触功能。
J Parkinsons Dis. 2015;5(4):699-713. doi: 10.3233/JPD-150642.
Zotero 插件