Farias C F, Azevedo R A, Brito-Gitirana L
Department of Histology and Embryology, Federal University of Rio de Janeiro, CEP 21540-970, Rio de Janeiro, RJ, Brazil.
Braz J Biol. 2006 Feb;66(1A):45-51. doi: 10.1590/s1519-69842006000100007. Epub 2006 May 2.
The Bidder's organ and ovary of the Brazilian toad Bufo ictericus were studied by light microscopy, using hematoxylin-eosin (HE) and periodic acid Schiff (PAS) staining. The expression and distribution of carbohydrate moieties was analyzed by lectin histochemistry, using 8 lectins with different carbohydrate specificities: Ulex europaeus (UEA I), Lens culinaris (LCA), Erythrina cristagalli (ECA), Arachis hypogaea (PNA), Ricinus communis (RCA I), Aleuria aurantia (AAA), Triticum vulgaris (WGA), and Glycine maximum (SBA). The results showed that the Bidderian zona pellucida presented alpha-mannose, alpha-L-fucose, beta-D-galactose, N-acetyl-D-glucosamine, and alpha/beta-N-acetyl-galactosamine residues. The Bidderian follicular cells showed the presence of beta-D-galactose and N-acetyl-D-glucosamine. In the extracellular matrix, alpha-mannose and alpha/beta-N-acetyl-galactosamine residues were detected. The ovarian zona pellucida showed alpha-L-fucose, N-acetyl-D-glucosamine, alpha/beta-N-acetyl-galactosamine residues, and alpha-mannose and N-acetyl-D-glucosamine residues were detected in the follicular cells. Thus, the zona pellucida in both organs contains N-acetyl-D-glucosamine, and alpha/beta-N-acetyl-galactosamine residues. alpha-L-fucose residues were detected in the zona pellucida of both organs, using different lectins. Considering that beta-D-galactose residue was absent from ovary but present in the Bidder's organ, this sugar residue may play an important role in follicle development, blocking the Bidderian follicles and preventing further development of the Bidder's organ into a functional ovary.
采用苏木精-伊红（HE）染色和过碘酸希夫（PAS）染色，通过光学显微镜对巴西蟾蜍Bufo ictericus的毕德器和卵巢进行了研究。利用8种具有不同碳水化合物特异性的凝集素，通过凝集素组织化学分析碳水化合物部分的表达和分布：欧洲荆豆（UEA I）、菜豆（LCA）、刺桐（ECA）、花生（PNA）、蓖麻（RCA I）、橙黄银耳（AAA）、普通小麦（WGA）和大豆（SBA）。结果表明，毕德器透明带呈现α-甘露糖、α-L-岩藻糖、β-D-半乳糖、N-乙酰-D-葡萄糖胺和α/β-N-乙酰半乳糖胺残基。毕德器卵泡细胞显示存在β-D-半乳糖和N-乙酰-D-葡萄糖胺。在细胞外基质中，检测到α-甘露糖和α/β-N-乙酰半乳糖胺残基。卵巢透明带显示α-L-岩藻糖、N-乙酰-D-葡萄糖胺、α/β-N-乙酰半乳糖胺残基，卵泡细胞中检测到α-甘露糖和N-乙酰-D-葡萄糖胺残基。因此，两个器官的透明带都含有N-乙酰-D-葡萄糖胺和α/β-N-乙酰半乳糖胺残基。使用不同的凝集素在两个器官的透明带中都检测到了α-L-岩藻糖残基。鉴于卵巢中不存在β-D-半乳糖残基，但在毕德器中存在，这种糖残基可能在卵泡发育中起重要作用，阻碍毕德器卵泡并阻止毕德器进一步发育成功能性卵巢。
