Sano H, Matsukubo T, Shibasaki K, Itoi H, Takaesu Y
Tokyo Dental College.
Bull Tokyo Dent Coll. 1991 Feb;32(1):9-17.
This study estimated the effects of five chitin derivatives low molecular chitosan (LMCS), ethyleneglycol chitin (PEGT), carboxymethyl chitin (PCMT), sulphated chitosan (PSSS), and phosphorylated chitin (PPPT) on the adsorption of three oral streptococci to saliva-treated hydroxyapatite (S-HA). The adsorption was evaluated by measuring the optical density of the bacterial cell suspensions released from saliva-treated hydroxyapatite by 0.5 N HCl. The adsorption of test strains to S-HA progressively decreased in proportion to each additional volume of PEGT, PPPT, or PSSS. PPPT and PSSS quite effectively inhibited the adsorption of S. mutans onto S-HA, but were less effective against S. sanguis and S. mitis. PPPT, PSSS, and PCMT all markedly promoted the desorption of S. mutans cells pre-adsorbed onto S-HA. Pretreatment of S-HA with PPPT, PSSS, or PCMT significantly decreased the subsequent adsorption of S. mutans and S. mitis. Pretreatment of these cells with PEGT also decreased their adsorption to S-HA. These findings suggest that these chitin derivatives may change the ionic natures of the S-HA and the bacterial cell surface, resulting in a less favorable interaction.
本研究评估了五种几丁质衍生物（低分子壳聚糖（LMCS）、乙二醇几丁质（PEGT）、羧甲基几丁质（PCMT）、硫酸化壳聚糖（PSSS）和磷酸化几丁质（PPPT））对三种口腔链球菌吸附于经唾液处理的羟基磷灰石（S-HA）的影响。通过测量经0.5 N盐酸从经唾液处理的羟基磷灰石释放的细菌细胞悬液的光密度来评估吸附情况。随着PEGT、PPPT或PSSS添加量的增加，测试菌株对S-HA的吸附呈比例逐渐降低。PPPT和PSSS相当有效地抑制了变形链球菌对S-HA的吸附，但对血链球菌和缓症链球菌的效果较差。PPPT、PSSS和PCMT均显著促进了预先吸附在S-HA上的变形链球菌细胞的解吸。用PPPT、PSSS或PCMT对S-HA进行预处理可显著降低随后变形链球菌和缓症链球菌的吸附。用PEGT对这些细胞进行预处理也会降低它们对S-HA的吸附。这些发现表明，这些几丁质衍生物可能会改变S-HA和细菌细胞表面的离子性质，从而导致相互作用不太有利。
