Cai S, Simionato M R, Mayer M P, Novo N F, Zelante F
Department of Microbiology, University of São Paulo, Brazil.
Caries Res. 1994;28(5):335-41. doi: 10.1159/000261998.
The aim of this investigation was to determine whether sublethal concentrations of chlorhexidine (Cx), hexetidine (Hx), cetylpyridinium chloride (Cc), sodium dodecyl sulfate (SDS), sanguinarine (Sg), sodium fluoride (NaF), and ammonium fluoride (NH4F) could affect hydrophobicity and adhesion of Streptococcus sanguis and Streptococcus mutans to saliva-coated hydroxyapatite (S-HA). Determination of the minimum inhibitory concentrations (MICs) showed that both species were susceptible to all agents tested. Growth in the presence of sub-MIC concentrations of Cx, SDS, Cc, NaF, or NH4F did not change significantly the hydrophobicity of S. sanguis cells when compared to the control which lacked any agent. However, growth in the presence of Hx or Sg resulted in a significant reduction in their hydrophobicity. Sub-MIC levels of SDS or Sg in the growth medium resulted in S. mutans cells with increased affinity for hexadecane compared with the control. The adherence of S. sanguis was changed significantly only by Hx or Sg, resulting in less cells adhering to S-HA. However, S. mutans cells previously incubated with NaF, NH4F, or Sg showed a higher adherence to S-HA than the control. The mechanisms of interference with adherence are at present not completely understood. Thus, antimicrobial agents at sub-MIC concentrations can interfere selectively with hydrophobicity and/or adhesion of oral streptococci.
本研究的目的是确定洗必泰（Cx）、己脒定（Hx）、西吡氯铵（Cc）、十二烷基硫酸钠（SDS）、血根碱（Sg）、氟化钠（NaF）和氟化铵（NH₄F）的亚致死浓度是否会影响血链球菌和变形链球菌对唾液包被的羟基磷灰石（S-HA）的疏水性和黏附性。最低抑菌浓度（MICs）的测定表明，这两种菌对所有测试药物均敏感。与不含任何药物的对照组相比，在亚MIC浓度的Cx、SDS、Cc、NaF或NH₄F存在下生长，血链球菌细胞的疏水性没有显著变化。然而，在Hx或Sg存在下生长会导致其疏水性显著降低。生长培养基中亚MIC水平的SDS或Sg会使变形链球菌细胞对十六烷的亲和力增加，与对照组相比。血链球菌的黏附性仅因Hx或Sg而发生显著变化，导致黏附到S-HA上的细胞减少。然而，先前用NaF、NH₄F或Sg孵育的变形链球菌细胞对S-HA的黏附性高于对照组。目前对黏附干扰机制尚未完全了解。因此，亚MIC浓度的抗菌药物可选择性地干扰口腔链球菌的疏水性和/或黏附性。
