Ferrer Angel, Carnés Jerónimo, Marco F M, Andréu Carmen, Fernández-Caldas Enrique
Allergy Department, Hospital de la Vega Baja, Orihuela, Alicante, Spain.
Ann Allergy Asthma Immunol. 2006 Apr;96(4):579-85. doi: 10.1016/S1081-1206(10)63553-3.
The development of sensitization and symptoms after the inhalation of epithelial allergens is common.
To investigate the allergic response (in vivo and in vitro) of 3 individuals clinically sensitive and occupationally exposed to cow and goat and to evaluate the allergenic cross-reactivity between cow and goat epithelium extracts.
Three patients--a butcher and 2 doctors in veterinary medicine--were evaluated. These patients reported allergic respiratory symptoms after occupational exposure to goats and cows. Extracts were prepared from epithelia of both animals. All the patients underwent organ-specific allergen challenges with cow and goat extracts. Four nasal and 2 bronchial challenges were conducted following standardized procedures.
All 3 patients had positive challenge results with the offending allergen extract. Specific IgE to goat allergens was detected in all patients. Several IgE-binding bands were detected. Inhibition assays (enzyme allergosorbent test and immunoblots) confirmed moderate-to-high cross-reactivity between goat and cow extracts.
We confirm clinical sensitivity and specific IgE binding to goat and cow allergens in occupationally exposed individuals. There was good correlation among the clinical history, exposure, and the laboratory findings.
吸入上皮过敏原后发生致敏和症状很常见。
调查3名临床上对牛和山羊敏感且职业性接触牛和山羊的个体的过敏反应（体内和体外），并评估牛和山羊上皮提取物之间的过敏原交叉反应性。
评估了3名患者——一名屠夫和2名兽医。这些患者报告在职业性接触山羊和牛后出现过敏性呼吸道症状。从两种动物的上皮制备提取物。所有患者均接受了牛和山羊提取物的器官特异性过敏原激发试验。按照标准化程序进行了4次鼻激发试验和2次支气管激发试验。
所有3名患者对致病过敏原提取物的激发试验结果均为阳性。在所有患者中均检测到针对山羊过敏原的特异性IgE。检测到几条IgE结合带。抑制试验（酶联免疫吸附试验和免疫印迹）证实山羊和牛提取物之间存在中度至高度交叉反应性。
我们证实了职业性接触个体对山羊和牛过敏原的临床敏感性以及特异性IgE结合。临床病史、接触情况和实验室检查结果之间存在良好的相关性。
