Pérez Eva, Blanco Carlos, Bartolomé Borja, Ortega Nancy, Castillo Rodolfo, Dumpiérrez Antonio G, Almeida Lourdes, Carrillo Teresa
Sección de Alergia, Hospital Universitario de Gran Canaria Dr Negrín, Las Palmas de Gran Canaria, Spain.
Ann Allergy Asthma Immunol. 2006 May;96(5):719-22. doi: 10.1016/S1081-1206(10)61071-X.
Acalypha wilkesiana, or copperleaf, is a plant of the Euphorbiaceae family. Although it is widely known as an outdoor ornamental plant, no cases of A. wilkesiana allergy have been reported to date.
To describe a patient with occupational respiratory allergy to A. wilkesiana.
Extracts from A. wilkesiana leaves and flowers were used for skin prick testing, specific conjunctival and bronchial challenge tests, and in vitro studies. These studies range from A. wilkesiana specific IgE determination to sodium dodecyl sulfate-polyacrylamide gel electrophoresis immunodetection of A. wilkesiana protein bands in patient serum samples and immunoblot inhibition by preincubation with Salsola kali and Chenopodium album pollen extracts.
Our patient had positive skin prick test reactions to A. wilkesiana leaf and flower extracts; negative reactions were found in a control group of 20 atopic patients. On immunodetection of A. wilkesiana extracts in patient serum samples, as many as 9 different IgE-binding proteins, with apparent molecular weights of 16 to 86 kDa, were revealed. Preincubation with S. kali and C. album pollen extracts completely inhibited IgE binding to the A. wilkesiana extract. Specific bronchial challenge resulted in a spirometric 30% decline in forced expiratory volume in 1 second with respect to baseline 1 minute after 1:100 (vol/vol) A. wilkesiana extract solution inhalation; 2 atopic controls had negative bronchial challenge test results.
Acalypha wilkesiana is a new etiologic agent for IgE-mediated occupational respiratory allergy.
红桑，又称铜叶植物，是大戟科的一种植物。尽管它作为一种户外观赏植物广为人知，但迄今为止尚未有红桑过敏病例的报道。
描述一名对红桑有职业性呼吸道过敏的患者。
使用红桑的叶和花提取物进行皮肤点刺试验、特异性结膜和支气管激发试验以及体外研究。这些研究包括测定红桑特异性IgE、对患者血清样本中红桑蛋白条带进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳免疫检测，以及用盐角草和藜花粉提取物预孵育进行免疫印迹抑制。
我们的患者对红桑叶和花提取物的皮肤点刺试验反应呈阳性；在20名特应性患者的对照组中发现为阴性反应。在对患者血清样本中的红桑提取物进行免疫检测时，发现多达9种不同的IgE结合蛋白，其表观分子量为16至86 kDa。用盐角草和藜花粉提取物预孵育可完全抑制IgE与红桑提取物的结合。特异性支气管激发试验在吸入1:100（体积/体积）红桑提取物溶液1分钟后，1秒用力呼气量相对于基线下降了30%；2名特应性对照者的支气管激发试验结果为阴性。
红桑是IgE介导的职业性呼吸道过敏的一种新的致病因素。
