Parke L, Forsyth I A
Endocr Res Commun. 1975;2(2):137-49. doi: 10.1080/07435807509053844.
Using 125-I-labelled ovine prolactin and receptors isolated from the livers of rabbits, a sensitive method has been developed suitable for the assay of ovine, bovine, porcine, human and rat prolactins. These hormones showed competitive displacement of 125-I-ovine prolactin which was in general agreement with their respective activities in the pigeon crop sac bioassay. Human and monkey growth hormones and human placental lactogen, which have marked prolactin-like actions on mammary tissue were also effective competitors. Non-primate growth hormones (ovine, bovine, equine and canine) which do not have prolactin-like activity gave little if any displacement as did human FSH, LH, TSH, ACTH and bovine insulin. Preparations of equine and canine prolactin of varying purity gave dose-response curves although their activity as competitors relative to ovine prolactin was poor and not related to their pigeon crop stimulating activity. This indicates species differences between prolactins in hormone-receptor interaction. Experiments with antiserum to human growth hormone have suggested an effective method of making the assay specific in species such as man in which prolactin is not the sole hormone with lactogenic activity.
利用从兔肝脏分离的125-I标记的绵羊催乳素和受体，已开发出一种灵敏的方法，适用于测定绵羊、牛、猪、人及大鼠的催乳素。这些激素显示出对125-I-绵羊催乳素的竞争性置换，这与它们在鸽嗉囊生物测定中的各自活性总体一致。对乳腺组织有显著催乳素样作用的人及猴生长激素和人胎盘催乳素也是有效的竞争者。不具有催乳素样活性的非灵长类生长激素（绵羊、牛、马和犬）几乎没有或根本没有置换作用，人促卵泡激素、促黄体激素、促甲状腺激素、促肾上腺皮质激素及牛胰岛素也是如此。不同纯度的马和犬催乳素制剂给出了剂量反应曲线，尽管它们作为相对于绵羊催乳素的竞争者的活性较差，且与它们刺激鸽嗉囊的活性无关。这表明催乳素在激素-受体相互作用方面存在种属差异。用人生长激素抗血清进行的实验提示了一种使该测定在人等物种中具有特异性的有效方法，在这些物种中催乳素不是具有生乳活性的唯一激素。
