兔组织中亚灵长类胎盘催乳素结合的定位与特异性
Localization and specificity of binding of subprimate placental lactogen in rabbit tissues.
作者信息
Bolander F F, Hurley T W, Handwerger S, Fellows R E
出版信息
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2932-5. doi: 10.1073/pnas.73.8.2932.
A search for specific placental lactogen binding was undertaken in tissues obtained from late pregnant rabbits using the placental lactogens from sheep, cows, and human beings. 125I-labeled ovine lactogen exhibited highest specific binding to the adrenal gland (57.8%), followed by liver (21.5%), ovary (19.9%), mammary gland (15.9%), uterus (12.2%), kidney (8.8%), brain (8.5%), and adipose tissue (7.9%). In liver and mammary gland, the displacement curves for ovine and human lactogen were identical to that for bovine prolactin, indicating that they share the same receptor site. Although the displacement curve for bovine lactogen was parallel to that of the other lactogens the bovine hormone is less active in the radioreceptor assay.
利用绵羊、牛和人的胎盘催乳素，对取自妊娠晚期兔子的组织进行了特定胎盘催乳素结合的研究。125I标记的绵羊催乳素与肾上腺的特异性结合最高（57.8%），其次是肝脏（21.5%）、卵巢（19.9%）、乳腺（15.9%）、子宫（12.2%）、肾脏（8.8%）、大脑（8.5%）和脂肪组织（7.9%）。在肝脏和乳腺中，绵羊和人催乳素的置换曲线与牛催乳素的置换曲线相同，表明它们共享相同的受体位点。虽然牛催乳素的置换曲线与其他催乳素的曲线平行，但在放射受体测定中，牛激素的活性较低。
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