Misty Russ, Martinez Raquel, Ali Hind, Steimle Paul A
Department of Biology, University of North Carolina at Greensboro, Greensboro, NC 27402, USA.
Biochem Biophys Res Commun. 2006 Jun 23;345(1):516-22. doi: 10.1016/j.bbrc.2006.04.047. Epub 2006 Apr 25.
Naringenin is a flavanone compound that alters critical cellular processes such as cell multiplication, glucose uptake, and mitochondrial activity. In this study, we used the social amoeba, Dictyostelium discoideum, as a model system for examining the cellular processes and signaling pathways affected by naringenin. We found that naringenin inhibited Dictyostelium cell division in a dose-dependent manner (IC(50) approximately 20 microM). Assays of Dictyostelium chemotaxis and multicellular development revealed that naringenin possesses a previously unrecognized ability to suppress amoeboid cell motility. We also found that naringenin, which is known to inhibit phosphatidylinositol 3-kinase activity, had no apparent effect on phosphatidylinositol 3,4,5-trisphosphate synthesis in live Dictyostelium cells; suggesting that this compound suppresses cell growth and migration via alternative signaling pathways. In another context, the discoveries described here highlight the value of using the Dictyostelium model system for identifying and characterizing the mechanisms by which naringenin, and related compounds, exert their effects on eukaryotic cells.
柚皮素是一种黄烷酮化合物，它能改变关键的细胞过程，如细胞增殖、葡萄糖摄取和线粒体活性。在本研究中，我们使用社会变形虫盘基网柄菌作为模型系统，来研究受柚皮素影响的细胞过程和信号通路。我们发现柚皮素以剂量依赖的方式抑制盘基网柄菌的细胞分裂（半数抑制浓度约为20微摩尔）。对盘基网柄菌趋化性和多细胞发育的测定表明，柚皮素具有一种以前未被认识到的抑制变形虫细胞运动的能力。我们还发现，已知能抑制磷脂酰肌醇3激酶活性的柚皮素，对活的盘基网柄菌细胞中磷脂酰肌醇3,4,5-三磷酸的合成没有明显影响；这表明该化合物通过替代信号通路抑制细胞生长和迁移。在另一种情况下，这里描述的发现突出了使用盘基网柄菌模型系统来识别和表征柚皮素及相关化合物对真核细胞发挥作用的机制的价值。
