Gemmell E, Seymour G J
Department of Dentistry, University of Queensland, Australia.
Oral Microbiol Immunol. 1991 Dec;6(6):356-62. doi: 10.1111/j.1399-302x.1991.tb00507.x.
B-cells extracted from periodontal disease tissue were analyzed for the presence of activation markers using a range of monoclonal antibodies. In adult periodontitis (AP), 6% of B-cells expressed the IL-2 receptor (CD25) compared with 1-2% in peripheral blood and healthy or marginal gingivitis (H/MG) gingival B-cells. There was also an increase in the mean percentage of IgD-positive B-cells and a decrease in CD21 and CD22 expression. In both AP and H/MG lesions, 20-22% of the B-cells expressed CD23 compared with less than 5% in peripheral blood. As B-cells are activated by day 3 in culture and start differentiating into immunoglobulin-secreting cells by day 6, B-cell phenotypes were assayed at these times in this study. Following stimulation with the periodontopathic bacterium Porphyromonas gingivalis, the expression of CD23, CD21 and CD22 on B-cells extracted from AP lesions remained relatively constant over the 6-d culture period. However, with Fusobacterium nucleatum stimulation, there was a significant decrease in CD23, CD21 and CD22 expression after 3 d in culture, which corresponds to the activation time for B-cells. These results show that B-cells extracted from periodontal disease tissue display a range of activation markers and on stimulation, demonstrate differing responses to individual periodontopathic bacteria.
使用一系列单克隆抗体分析从牙周疾病组织中提取的B细胞，以检测激活标志物的存在情况。在成人牙周炎（AP）中，6%的B细胞表达白细胞介素-2受体（CD25），而外周血以及健康或边缘性牙龈炎（H/MG）牙龈B细胞中的这一比例为1%-2%。IgD阳性B细胞的平均百分比也有所增加，而CD21和CD22的表达则有所下降。在AP和H/MG病变中，20%-22%的B细胞表达CD23，而外周血中这一比例不到5%。由于B细胞在培养3天时被激活，并在6天时开始分化为分泌免疫球蛋白的细胞，因此在本研究中于这些时间点检测了B细胞表型。用牙周病原菌牙龈卟啉单胞菌刺激后，从AP病变中提取的B细胞上CD23、CD21和CD22的表达在6天培养期内保持相对恒定。然而，在用具核梭杆菌刺激后，培养3天后CD23、CD21和CD22的表达显著下降，这与B细胞的激活时间相对应。这些结果表明，从牙周疾病组织中提取的B细胞表现出一系列激活标志物，并且在受到刺激后，对不同的牙周病原菌表现出不同的反应。
