Parkes A B, Williams S, Howells R D, Harris R, Lazarus J H, Waters J S, Hall R
Department of Medicine, University of Wales College of Medicine, Cardiff, UK.
J Clin Lab Immunol. 1991 May;35(1):1-7.
This paper describes the use of sensitized sheep red blood cells for the detection and titration of complement fixation by autoantibodies directed against human thyroid membranes in the serum of patients with autoimmune thyroid disease. Patients with elevated circulating levels of TPO antibodies and diagnosed as having autoimmune hypothyroidism (including Hashimoto's disease) or autoimmune hyperthyroidism (Graves' disease) were studied. Complement fixation titres were highest in those patients with autoimmune hypothyroidism compared with the autoimmune hyperthyroid group. Serum samples obtained from a group of patients with thyroid neoplasia and from normal healthy volunteers were negative in this test. The TPO antibody activity when "corrected" for its CF potency suggests that the autoantibodies found in autoimmune hypothyroidism are potentially more destructive than those found in the non-destructive autoimmune thyroid diseases.
本文描述了利用致敏绵羊红细胞检测和滴定自身免疫性甲状腺疾病患者血清中针对人甲状腺膜的自身抗体的补体结合情况。研究对象为循环中TPO抗体水平升高且被诊断为自身免疫性甲状腺功能减退（包括桥本氏病）或自身免疫性甲状腺功能亢进（格雷夫斯病）的患者。与自身免疫性甲状腺功能亢进组相比，自身免疫性甲状腺功能减退患者的补体结合滴度最高。从一组甲状腺肿瘤患者和正常健康志愿者获得的血清样本在该检测中呈阴性。对TPO抗体活性进行“校正”以考虑其补体结合效力后表明，自身免疫性甲状腺功能减退中发现的自身抗体可能比非破坏性自身免疫性甲状腺疾病中发现的自身抗体更具破坏性。
