Hopf U, Fiek T, Grün R, Zuschneid W
J Clin Lab Immunol. 1982 Oct;9(1):49-53.
Antibodies against the plasma membrane of isolated human and porcine thyroid cells were demonstrable by a linear fluorescence pattern in sera from 29 out of 37 patients with Graves' disease. In 3 cases the antibodies reacted only with human thyroid cells. In a group of patients with Hashimoto's thyroiditis, 6 of 8 sera reacted with human thyroid cells in a granular fluorescence pattern. With one exception, sera from patients with non-toxic goiter showed no binding of IgG to thyroid cells. Sera from healthy controls were negative in this test system. There was no correlation between the presence of antibodies against thyroid cells and antibodies against thyroglobulin. Antibodies against thyroid microsomal antigens were found in 80% of patients with circulating thyroid cell membrane antibodies; 5 sera with membrane antibodies were negative for microsomal antibodies. Twenty sera of patients with Graves' disease were tested by passive haemagglutination test against peak I and peak II of the human thyroid fractionation with Sephadex G100. Antibodies against peak I were found in 14 sera and against peak II in all 20 sera. The data indicate that the antibodies in sera from patients with Graves' disease are directed mainly against a species-unspecific antigen of the plasma membrane from thyroid cells with a probable molecular weight between 4-7 S and are unrelated to thyroglobulin.
在37例格雷夫斯病患者中，29例患者血清中的线性荧光模式可证实存在针对分离出的人及猪甲状腺细胞质膜的抗体。3例患者的抗体仅与人甲状腺细胞发生反应。在一组桥本甲状腺炎患者中，8份血清中有6份以颗粒状荧光模式与人甲状腺细胞发生反应。除1例例外，非毒性甲状腺肿患者的血清在该检测系统中未显示IgG与甲状腺细胞结合。健康对照者的血清在该检测系统中呈阴性。针对甲状腺细胞的抗体与抗甲状腺球蛋白抗体之间无相关性。在80%循环甲状腺细胞膜抗体阳性的患者中发现了抗甲状腺微粒体抗原的抗体；5份有膜抗体的血清微粒体抗体呈阴性。用被动血凝试验对20例格雷夫斯病患者的血清进行检测，以检测其针对用葡聚糖凝胶G100进行人甲状腺分级分离的峰I和峰II的抗体。14份血清中发现了针对峰I的抗体，20份血清中均发现了针对峰II的抗体。数据表明，格雷夫斯病患者血清中的抗体主要针对甲状腺细胞质膜的一种种属非特异性抗原，其分子量可能在4 - 7S之间，且与甲状腺球蛋白无关。
