Quaglia M G, Desideri N, Bossù E, Melchiorre P, Conti C
Dipartimento di Studi Farmaceutici, Università, La Sapienza, Rome, Italy.
J Pharm Biomed Anal. 1991;9(10-12):811-5. doi: 10.1016/0731-7085(91)80006-u.
A variety of racemic flavanoids with anti-rhinovirus activity have been resolved for the first time by HPLC, using a chiral stationary phase. Baseline separation was easily obtained for racemic 4',6-dichloroisoflavan (V). The absolute configurations of two enantiomers (Va and Vb) were established by comparing their circular dichroism curves with those of other known isoflavans. Both the isomers were tested against human rhinovirus serotype 1B infection in vitro; the S form was approximately four times more effective than the R form.
首次使用手性固定相通过高效液相色谱法拆分了多种具有抗鼻病毒活性的外消旋类黄酮。外消旋4',6-二氯异黄酮（V）很容易实现基线分离。通过将两种对映体（Va和Vb）的圆二色曲线与其他已知异黄酮的曲线进行比较，确定了它们的绝对构型。两种异构体均在体外针对人鼻病毒1B型感染进行了测试；S型的效果约为R型的四倍。
