Gerken G, Kremsdorf D, Petit M A, Manns M, Meyer zum Büschenfelde K H, Bréchot C
INSERM Unité 75, CHU Necker, Clamart, France.
J Hepatol. 1991;13 Suppl 4:S93-6. doi: 10.1016/0168-8278(91)90034-9.
We have found a defective form of hepatitis B in an anti-HBe positive chronic HBsAg carrier with liver cancer (HCC). Viral deletions were identified in the preS coding region using PCR and sequencing. The presence of deleted HBV forms was observed in serum, PBMC, and liver tissue. To conclude, rearrangements and deletions in the preS gene would potentially lead to an impairment in viral clearance without affecting viral penetration in liver cells, possibly accounting for chronic HBV infection.
我们在一名患有肝癌（HCC）的抗-HBe阳性慢性HBsAg携带者中发现了一种缺陷形式的乙型肝炎病毒。使用聚合酶链反应（PCR）和测序技术在preS编码区鉴定出病毒缺失。在血清、外周血单个核细胞（PBMC）和肝组织中观察到缺失的HBV形式的存在。总之，preS基因的重排和缺失可能会导致病毒清除受损，而不影响病毒在肝细胞中的渗透，这可能是慢性HBV感染的原因。
