CK34BE12联合蛋白P53基因表达及前列腺特异性抗原在前列腺癌鉴别诊断中的临床价值
[Clinical value of CK34BE12 combining the expression of protein P53 gene and prostate specific antigen for the differential diagnosis of prostate carcinoma].
作者信息
Gu Xiao-jian, Lu Jian-lin, Lai Ren-sheng, Zhang Ya-da, Zhang Ping, Lu Zi-jie, Zhu Qing-yi
机构信息
Department of Urology, Jiangsu Hospital of TCM, Nanjing, China.
出版信息
Zhonghua Nan Ke Xue. 2006 Apr;12(4):340-2.
OBJECTIVE
To improve the level of clinical diagnosis and differential diagnosis of benign and malignant prostate lesions.
METHODS
One hundred and nine cases of prostate cancer and prostate hyperplasia were evaluated by the expression of high molecular weight cytokeratin (CK34BE12), prostate specific antigen (PSA) and protein P53 gene using the immunohistochemical technique.
RESULTS
The basal-cells in all of the benign lesions were stained with the CK34BE12 and PSA, while it had not immunoreactivity with P53. In contrast, the prostate carcinoma were not stained or partly stained with the CK34BE12 and PSA, but P53 show significant immunoreactivity with the tissue.
CONCLUSION
Based on the routine histological studies with the expression of CK34BE12 and PSA together, they can indicate the existence of basal-cell distinctly and show indirectly whether the basal-cell is integrated. Combining the expression of P53 to determine the existence of cancer gene, it can help to distinguish benign and malignant prostate lesions.
目的
提高前列腺良恶性病变的临床诊断及鉴别诊断水平。
方法
采用免疫组化技术，通过高分子量细胞角蛋白（CK34BE12）、前列腺特异性抗原（PSA）及蛋白P53基因的表达情况，对109例前列腺癌及前列腺增生患者进行评估。
结果
所有良性病变的基底细胞均被CK34BE12和PSA染色，而与P53无免疫反应性。相比之下，前列腺癌组织对CK34BE12和PSA无染色或部分染色，但P53与组织呈现明显的免疫反应性。
结论
基于常规组织学研究，联合CK34BE12和PSA的表达情况，可清晰显示基底细胞的存在，并间接表明基底细胞是否完整。结合P53的表达来判断癌基因的存在，有助于鉴别前列腺良恶性病变。
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