DNA中胸腺嘧啶的三重态能量。
The triplet energy of thymine in DNA.
作者信息
Bosca Francisco, Lhiaubet-Vallet Virginie, Cuquerella M Consuelo, Castell Jose V, Miranda Miguel A
机构信息
Instituto de Tecnología Química UPV-CSIC, Universidad Politécnica de Valencia, Avda de los Naranjos s/n, 46022 Valencia, Spain.
出版信息
J Am Chem Soc. 2006 May 17;128(19):6318-9. doi: 10.1021/ja060651g.
Norfloxacin (NFX) photosensitizes formation of thymine dimers (T<>T) in DNA, while its N(4') acetyl derivative (ANFX) does not. This is evident from the observation of single-strand breaks after enzymatic treatment with T4 endonuclease V and subsequent gel electrophoresis. The triplet energies of NFX and ANFX are estimated at 273 and 268 kJ/mol, respectively, on the basis of triplet-triplet energy transfer quenching by a set of biphenyl and naphthalene derivatives. Hence, the triplet energy of thymine in DNA (i.e., the value for a photosensitizer to produce T<>T) can be estimated at 270 kJ/mol.
诺氟沙星（NFX）可使DNA中胸腺嘧啶二聚体（T<>T）光致敏形成，而其N(4')-乙酰衍生物（ANFX）则不会。在用T4内切核酸酶V进行酶处理并随后进行凝胶电泳后观察到的单链断裂现象，证明了这一点。基于一组联苯和萘衍生物的三重态-三重态能量转移猝灭，估计NFX和ANFX的三重态能量分别为273和268 kJ/mol。因此，DNA中胸腺嘧啶的三重态能量（即产生T<>T的光敏剂的值）估计为270 kJ/mol。
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