Hård K, Mekking A, Kamerling J P, Dacremont G A, Vliegenthart J F
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Glycoconj J. 1991 Feb;8(1):17-28. doi: 10.1007/BF00731639.
Five brain-derived and 17 urinary oligomannose-type oligosaccharides were isolated by ion-exchange chromatography on Mono Q or Dowex, followed by HPLC on Lichrosorb-NH2 from a Persian cat suffering from alpha-mannosidosis. The structures of the carbohydrate chains were determined by 500- or 600-MHz 1H-NMR spectroscopy. Different oligosaccharide patterns were found in brain and urine. 99% of the urinary oligosaccharides possess an alpha(1-6)-linked mannose residue attached to beta-mannose, whereas only 5% of the brain-derived oligosaccharides contain such a residue. Furthermore, of the urinary carbohydrate chains 71% end with Man beta 1-4GlcNAc beta 1-4GlcNAc and 29% end with Man beta 1-4GlcNAc, whereas the corresponding amounts are 23% and 77%, respectively, for the brain-derived oligosaccharides.
通过在Mono Q或Dowex上进行离子交换色谱，然后在Lichrosorb-NH2上进行高效液相色谱，从一只患有α-甘露糖苷贮积症的波斯猫中分离出5种脑源性和17种尿源性低聚甘露糖型寡糖。碳水化合物链的结构通过500兆赫或600兆赫的1H-核磁共振光谱确定。在脑和尿中发现了不同的寡糖模式。99%的尿源性寡糖具有连接到β-甘露糖上的α(1-6)-连接的甘露糖残基，而只有5%的脑源性寡糖含有这样的残基。此外，尿源性碳水化合物链中71%以Manβ1-4GlcNAcβ1-4GlcNAc结尾，29%以Manβ1-4GlcNAc结尾，而脑源性寡糖的相应比例分别为23%和77%。
