Rossi Gian Paolo, Sticchi Daniele, Giuliani Luisa, Bernante Paolo, Zavattiero Silvia, Pessina Achille C, Nussdorfer Gastone G
Department of Clinical and Experimental Medicine, University of Padua, Italy.
Int J Mol Med. 2006 Jun;17(6):975-80.
Adiponectin is an adipocyte-derived circulating peptide that plays an important role in adipose tissue metabolism, insulin sensitivity and cardiovascular disease. The adrenal gland, by secreting glucorticoid and mineralocorticoid hormones, intervenes in cardiovascular and glucose metabolism regulation and is surrounded by adipose tissue. Hence, we investigated the hypothesis that adiponectin receptor types 1 and 2 (adipo-R1 and adipo-R2) are expressed in the human adrenal gland and in adrenocortical zona glomerulosa cell-derived aldosterone-producing adenoma (APA) tissue. We used real-time reverse transcription-polymerase chain reaction to demonstrate the mRNA of adipo-R1 and adipo-R2 in 10 histologically normal human adrenal cortexes that were obtained from patients with renal cancer undergoing nephrectomy with ipsilateral adrenalectomy and in 10 APAs. Melting curve analysis and sequencing were used to confirm the specificity of the amplicons obtained. Results consistently showed the expression of specific mRNAs of adiponectin receptors in all histologically normal human adrenal cortexes and APAs. This novel finding suggests that adiponectin could play a regulatory role in adrenocortical function and growth in humans.
脂联素是一种由脂肪细胞分泌的循环肽，在脂肪组织代谢、胰岛素敏感性和心血管疾病中发挥重要作用。肾上腺通过分泌糖皮质激素和盐皮质激素来干预心血管和葡萄糖代谢调节，且被脂肪组织所包围。因此，我们研究了以下假说：脂联素受体1型和2型（Adipo-R1和Adipo-R2）在人肾上腺以及肾上腺皮质球状带细胞来源的醛固酮瘤（APA）组织中表达。我们使用实时逆转录聚合酶链反应来证实从接受肾切除术并同侧肾上腺切除术的肾癌患者获取的10个组织学正常的人肾上腺皮质以及10个APA中存在Adipo-R1和Adipo-R2的mRNA。熔解曲线分析和测序用于确认所获扩增子的特异性。结果一致显示，在所有组织学正常的人肾上腺皮质和APA中均存在脂联素受体特异性mRNA的表达。这一新发现表明脂联素可能在人类肾上腺皮质功能和生长中发挥调节作用。
