Park Bo-Young, Min Byung-Sun, Oh Sei-Ryang, Kim Jung-Hee, Bae Ki-Hwan, Lee Hyeong-Kyu
Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology, 52 Oundong, Yusong, Daejeon 305-333, Korea.
Phytother Res. 2006 Jul;20(7):610-3. doi: 10.1002/ptr.1915.
As part of an ongoing study aimed at identifying the anti-complement active compound from the flower buds of Daphne genkwa, ten flavonoids, one biscoumarin and one amide were isolated. Their structures were identified from spectroscopic and physicochemical data as genkwanin 5-O-beta-d-primveroside (1), apigenin 7-O-beta-d-glucuronide (2), genkwanin 5-O-beta-d-glucopyranoside (3), apigenin 5-O-beta-d-glucopyranoside (4), genkwanin (5), apigenin (6), luteolin 7-methyl ether (7), luteolin (8), daphnoretin (9), velutin (10), 7-methoxyacacetin (11) and aurantiamide acetate (12). Among them, compounds 9, 10 and 12 were isolated from this plant for the first time. The anti-complement activity of these compounds was tested against the classical pathway of the complement system. Among the compounds, daphnoretin (9) exhibited significant anti-complement activity with an IC(50) value of 11.4 microm, whereas the other compounds were not active in the assay.
作为一项旨在从芫花的花蕾中鉴定抗补体活性化合物的正在进行的研究的一部分，分离出了十种黄酮类化合物、一种双香豆素和一种酰胺。根据光谱和物理化学数据确定它们的结构分别为芫花苷5-O-β-d-樱草糖苷（1）、芹菜素7-O-β-d-葡萄糖醛酸苷（2）、芫花苷5-O-β-d-葡萄糖吡喃糖苷（3）、芹菜素5-O-β-d-葡萄糖吡喃糖苷（4）、芫花苷（5）、芹菜素（6）、木犀草素7-甲醚（7）、木犀草素（8）、瑞香素（9）、紫丁香苷（10）、7-甲氧基刺槐素（11）和醋酸橙酰胺（12）。其中，化合物9、10和12是首次从该植物中分离得到。测试了这些化合物针对补体系统经典途径的抗补体活性。在这些化合物中，瑞香素（9）表现出显著的抗补体活性，IC(50)值为11.4微摩尔，而其他化合物在该测定中无活性。
