Shaari Jeffrey, Palmer James N, Chiu Alexander G, Judy Kevin D, Cohen Akiva S, Kennedy David W, Cohen Noam A
Department of Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Am J Rhinol. 2006 Mar-Apr;20(2):150-4.
Mucociliary clearance, a primary host defense mechanism, depends on mucus production and its clearance by the coordinated beating of cilia lining the airways. Numerous investigations have analyzed ciliary activity in brushings from the inferior turbinate. To date, only one study has investigated whether there exists variation in ciliary beat frequency (CBF) within the sinonasal cavity. We analyzed CBF from the inferior turbinate, uncinate process, and sphenoethmoid recess in nonsinusitis patients to determine regional variability of ciliary activity within the sinonasal cavity.
Explants of sinonasal epithelium were analyzed at 37 degrees C. Beating cilia were visualized with differential interference contrast microscopy. Images were captured using a high-speed digital camera with a sampling rate of 250 frames per second. A one-dimensional tracking algorithm analyzed individual pixel grayscale values within each frame of the video. The differences in grayscale were plotted as a time-dependent waveform, and frequency was calculated as the inverse of the peak-to-peak distance. A minimum of three areas of beating cilia were analyzed per regional sample. Statistical analysis was performed with repeated-measures analysis of variance.
Complete sampling of all three sites was accomplished in 10 patients. No difference in CBF within the sinonasal cavity was identified (p < 0.05). The mean CBF for all sites in all patients was 12.6 +/- 2.9 Hz, in agreement with published values.
This study shows no regional differences in CBF within the sinonasal cavity, supporting previous work and validating analysis of inferior turbinate cilia.
黏液纤毛清除功能是一种主要的宿主防御机制，它依赖于黏液的产生以及气道内衬纤毛的协调摆动对其进行清除。众多研究分析了下鼻甲刷片中的纤毛活性。迄今为止，仅有一项研究调查了鼻窦腔内纤毛摆动频率（CBF）是否存在差异。我们分析了非鼻窦炎患者下鼻甲、钩突和蝶筛隐窝的CBF，以确定鼻窦腔内纤毛活性的区域变异性。
在37摄氏度下分析鼻窦上皮外植体。用微分干涉相差显微镜观察摆动的纤毛。使用采样率为每秒250帧的高速数码相机拍摄图像。一维跟踪算法分析视频每一帧内的单个像素灰度值。灰度差异被绘制成时间相关的波形，频率计算为峰峰距离的倒数。每个区域样本至少分析三个纤毛摆动区域。采用重复测量方差分析进行统计分析。
10例患者完成了所有三个部位的完整采样。未发现鼻窦腔内CBF有差异（p < 0.05）。所有患者所有部位的平均CBF为12.6 +/- 2.9 Hz，与已发表的值一致。
本研究表明鼻窦腔内CBF无区域差异，支持先前的研究工作并验证了下鼻甲纤毛分析。
