Gimelbrant Alexander A, Chess Andrew
Center for Human Genetic Research, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114, USA.
Genome Res. 2006 Jun;16(6):723-9. doi: 10.1101/gr.5023706. Epub 2006 May 10.
Asynchronous DNA replication is an epigenetically determined feature found in all cases of monoallelic expression, including genomic imprinting, X-inactivation, and random monoallelic expression of autosomal genes such as immunoglobulins and olfactory receptor genes. Most genes of the latter class were identified in experiments focused on genes functioning in the chemosensory and immune systems. We performed an unbiased survey of asynchronous replication in the mouse genome, excluding known asynchronously replicated genes. Fully 10% (eight of 80) of the genes tested exhibited asynchronous replication. A common feature of the newly identified asynchronously replicated areas is their proximity to areas of tandem gene duplication. Testing of other clustered areas supported the idea that such regions are enriched with asynchronously replicated genes.
异步DNA复制是一种表观遗传决定的特征,存在于所有单等位基因表达的情况中,包括基因组印记、X染色体失活以及常染色体基因(如免疫球蛋白和嗅觉受体基因)的随机单等位基因表达。后一类中的大多数基因是在专注于化学感应和免疫系统中发挥作用的基因的实验中被鉴定出来的。我们对小鼠基因组中的异步复制进行了无偏倚调查,排除了已知的异步复制基因。所测试的基因中有整整10%(80个中的8个)表现出异步复制。新鉴定出的异步复制区域的一个共同特征是它们靠近串联基因重复区域。对其他聚集区域的测试支持了这样一种观点,即这些区域富含异步复制基因。