Deltour L, Vandamme J, Jouvenot Y, Duvillié B, Kelemen K, Schaerly P, Jami J, Paldi A
Département Génétique, Développement et Pathologie Moléculaire, Institut Cochin, Inserm U567, CNRS UMR 8104, Université René Descartes, 75014 Paris, France.
Gene Expr Patterns. 2004 Dec;5(2):297-300. doi: 10.1016/j.modgep.2004.04.013.
There are two functional insulin genes in the mouse genome. The Ins2 gene is imprinted and expressed monoallelically from the paternal allele in the yolk sac. In the present study we have re-examined the imprinting status of Ins1. We found that Ins1 is not expressed in the yolk sac of several laboratory mouse strains. The asynchrony of replication at the wild type locus was significantly lower than at imprinted loci and was more similar to non-imprinted loci. Finally, we have taken the advantage of the Ins1(neo) allele created by homologous recombination to examine the allelic usage at this locus. We observed that the neo gene inserted at the Ins1 locus was expressed from both the paternally and the maternally transmitted allele. Therefore, the Ins1 gene does not share any of the basic properties of imprinted genes. On the basis of these data, we concluded that Ins1 locus is unlikely to be imprinted in common laboratory mice.
小鼠基因组中有两个功能性胰岛素基因。Ins2基因是印记基因，在卵黄囊中从父本等位基因单等位表达。在本研究中，我们重新检查了Ins1的印记状态。我们发现Ins1在几种实验室小鼠品系的卵黄囊中不表达。野生型位点的复制异步性明显低于印记位点，且与非印记位点更相似。最后，我们利用同源重组产生的Ins1(neo)等位基因来检测该位点的等位基因使用情况。我们观察到插入Ins1位点的neo基因从父本和母本传递的等位基因均有表达。因此，Ins1基因不具备印记基因的任何基本特性。基于这些数据，我们得出结论，Ins1位点在普通实验室小鼠中不太可能是印记的。
