Lemus-Varela M L, Sola A, Gómez-Meda B C, Zamora-Perez A L, Ramos-Ibarra M L, Batista-González C M, Zúñiga-González G M
Departamento de Neonatología, Hospital de Pediatría, Unidad Médica de Alta Especialidad, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México.
J Perinatol. 2006 Jul;26(7):423-7. doi: 10.1038/sj.jp.7211518. Epub 2006 May 4.
To determine sildenafil citrate (SC) genotoxicity and cytotoxicity in the Callithrix jacchus.
Fifteen organisms were assigned to one of three groups as follows: experimental (25 mg/kg of SC); negative control (glucose solution 5%); and positive control (3 mg/kg of cytocine arabinoside). Systemic hemodynamic changes were monitored in each animal before and after each treatment. A drop of blood was obtained before and after the treatment at 24-120 h. Smears were made and the frequency of micronucleated erythrocytes (MNE), micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes (PCE) was counted.
No significant differences in MNE, MNPCE and PCE were found in the group that received sildenafil and negative control. A significant increase in genotoxicity and cytotoxicity was observed in the positive control group. No changes were observed in systemic hemodynamic changes.
The macro-dose of SC lacks genotoxic, cytotoxic or systemic hemodynamic changes effects in this species.
确定枸橼酸西地那非（SC）对普通狨猴的遗传毒性和细胞毒性。
将15只动物分为以下三组之一：实验组（25mg/kg的SC）；阴性对照组（5%葡萄糖溶液）；阳性对照组（3mg/kg阿糖胞苷）。在每次治疗前后监测每只动物的全身血流动力学变化。在治疗后24 - 120小时采集一滴血。制作涂片并计数微核红细胞（MNE）、微核多染红细胞（MNPCE）和多染红细胞（PCE）的频率。
接受西地那非的组与阴性对照组在MNE、MNPCE和PCE方面未发现显著差异。阳性对照组观察到遗传毒性和细胞毒性显著增加。全身血流动力学变化未观察到改变。
该物种中SC的大剂量缺乏遗传毒性、细胞毒性或全身血流动力学变化影响。
