Alvarez M, Marrero M, Valdivia A, Garcia S, Valdes O, Morier L
Departamento de Virología, Instituto de Medicina Tropical Pedro Kouri, Siboney, Ciudad Habana, Cuba.
Rev Inst Med Trop Sao Paulo. 1991 May-Jun;33(3):175-9.
An Immunoperoxidase assay was applied to detect early antigens of cytomegalovirus (CMV) in 150 urine samples from immunocompromised patients, using the commercial available monoclonal antibody against CMV E13. The detection of early antigen by IP (IPCMV) is compared to the conventional cell culture isolation regarding specificity and sensitivity in order to evaluate is usefulness in the diagnostic of CMV infections. The IPCMV showed a sensitivity of 89.8% and a specificity of 91.3% when compared to the isolation method. The great advantage of the IPCMV is based on the shorter time results are achieved, since 48-72 Hs can be enough to provide evidence of CMV infection, while in the isolation technique cytopathogenic effect was present around 14 days after sample inoculation.
应用免疫过氧化物酶测定法，使用市售的抗巨细胞病毒（CMV）E13单克隆抗体，检测150份免疫功能低下患者尿液样本中的CMV早期抗原。将免疫过氧化物酶法检测早期抗原（IPCMV）与传统细胞培养分离法在特异性和敏感性方面进行比较，以评估其在CMV感染诊断中的实用性。与分离法相比，IPCMV的敏感性为89.8%，特异性为91.3%。IPCMV的一大优势在于获得结果的时间更短，因为48至72小时就足以提供CMV感染的证据，而在分离技术中，样本接种后约14天才会出现细胞病变效应。
