Guan Cui Ping, Jiang Zhen Ran, Zhou Yan Hong
Hubei Bioinformatics and Molecular Imaging Key Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China.
Genomics Proteomics Bioinformatics. 2005 Nov;3(4):247-51. doi: 10.1016/s1672-0229(05)03035-4.
G-protein coupled receptors (GPCRs) represent one of the most important classes of drug targets for pharmaceutical industry and play important roles in cellular signal transduction. Predicting the coupling specificity of GPCRs to G-proteins is vital for further understanding the mechanism of signal transduction and the function of the receptors within a cell, which can provide new clues for pharmaceutical research and development. In this study, the features of amino acid compositions and physiochemical properties of the full-length GPCR sequences have been analyzed and extracted. Based on these features, classifiers have been developed to predict the coupling specificity of GPCRs to G-proteins using support vector machines. The testing results show that this method could obtain better prediction accuracy.
G蛋白偶联受体(GPCRs)是制药行业最重要的一类药物靶点之一,在细胞信号转导中发挥着重要作用。预测GPCRs与G蛋白的偶联特异性对于进一步理解信号转导机制以及细胞内受体的功能至关重要,可为药物研发提供新线索。在本研究中,对全长GPCR序列的氨基酸组成和理化性质特征进行了分析和提取。基于这些特征,利用支持向量机开发了分类器来预测GPCRs与G蛋白的偶联特异性。测试结果表明,该方法能够获得较好的预测准确率。