Choi Soo Jung, Kim Mi Jeong, Heo Ho Jin, Kim Hye Kyung, Hong Bumshik, Kim Chang-Ju, Kim Byung-Gee, Shin Dong Hoon
Department of Food Technology, Korea University, Seongbuk-gu, Seoul, 136-701, Republic of Korea.
Amyloid. 2006 Mar;13(1):6-12. doi: 10.1080/13506120500535636.
The amyloid beta (Abeta) peptide is known to increase free radical production in nerve cells, leading to cell death. To investigate the effect of Rosa laevigata against Abeta-induced oxidative damage, in vitro assays and in vivo behavioral tests were performed. R. laevigata showed cell protective effects against oxidative stress-induced cytotoxicity. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction assay exhibited significant increase in cell viability when rat pheochromocytoma (PC 12) cells were treated with R. laevigata extracts. Administration of R. laevigata extracts to mice significantly reversed the Abeta-induced learning and memory impairment in in vivo behavioral tests. These results suggest that R. laevigata extracts can reduce the cytotoxicity of Abeta in PC 12 cells, possibly by the reduction of oxidative stress, and these extracts may be useful in the prevention of Alzheimer's disease.
已知β-淀粉样蛋白（Aβ）肽会增加神经细胞中的自由基产生，导致细胞死亡。为了研究金樱子对Aβ诱导的氧化损伤的影响，进行了体外试验和体内行为测试。金樱子对氧化应激诱导的细胞毒性具有细胞保护作用。当用金樱子提取物处理大鼠嗜铬细胞瘤（PC 12）细胞时，MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑）还原试验显示细胞活力显著增加。在体内行为测试中，给小鼠施用金樱子提取物可显著逆转Aβ诱导的学习和记忆障碍。这些结果表明，金樱子提取物可能通过降低氧化应激来降低Aβ在PC 12细胞中的细胞毒性，并且这些提取物可能有助于预防阿尔茨海默病。
