Research Institute of Biotechnology, HungKuang University, 34 Chung-Chie Rd, Sha Lu, Taichung 43302, Taiwan.
Evid Based Complement Alternat Med. 2012;2012:125247. doi: 10.1155/2012/125247. Epub 2011 Jul 5.
The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit of Terminalia chebula Retzius. The water extract afforded the greatest yield, and total phenolic and tannin content. The methanol extract yielded the greatest total triterpenoid content. Based on four chemiluminescence antioxidant assays, the three extracts showed various degrees of antioxidant activity. The methanol extract showed good antioxidant activity based on the horseradish peroxidase-luminol-hydrogen peroxide (H(2)O(2)) assay. The water extract appeared to have good antioxidant activities in cupric sulfate-Phen-Vc-H(2)O(2) and luminol-H(2)O(2) assays. Pyrogallol-luminol assay showed the 95% ethanol extract to have good antioxidant activity. The methanol and water extracts presented neuroprotective activities on H(2)O(2)-induced PC12 cell death at 0.5-5.0 μg/mL. Further investigations are necessary to verify these activities in vivo.
本研究旨在确定诃子（Terminalia chebula Retzius）干果实的水提物、甲醇提物和 95%乙醇提物的化学成分、化学发光抗氧化活性和对 PC12 细胞的神经保护作用。水提取物产率最高，总酚和单宁含量最高。甲醇提取物总三萜含量最高。基于四种化学发光抗氧化测定法，三种提取物表现出不同程度的抗氧化活性。甲醇提取物在辣根过氧化物酶-发光氨-过氧化氢（H2O2）测定法中表现出良好的抗氧化活性。水提取物在硫酸铜-邻苯三酚-Vc-H2O2 和发光氨-H2O2 测定法中似乎具有良好的抗氧化活性。邻苯三酚-发光氨测定法显示 95%乙醇提取物具有良好的抗氧化活性。甲醇和水提取物在 0.5-5.0μg/mL 浓度下对 H2O2 诱导的 PC12 细胞死亡具有神经保护作用。需要进一步的研究来验证这些在体内的活性。
