Gruys E, Ultee A, Upragarin N
Department of Pathobiology, Faculty of Veterinary Medicine Yalelaan, Utrecht University, Netherlands.
Amyloid. 2006 Mar;13(1):13-9. doi: 10.1080/13506120500535768.
In domestic brown layer fowl, reactive amyloidosis of internal organs, such as liver and spleen, and of the joints is a common disorder. In a variety of amyloid types including the AA-amyloid of the chicken, in addition to amyloid fibrils, proteoglycans and glycosaminoglycans (GAGs) are found on immunohistochemistry or after extraction. The aim of the present report is to study amyloid fibrils for the ultrastructural location of GAGs by cuprolinic blue staining and immunogold labeling. Rabbit antichicken AA antiserum was used for the immunogold labeling on conventionally embedded and cryoembedded liver tissue and revealed similar results. Therefore conventional blocks could be used for further analysis. Cuprolinic blue staining was performed on blocks of joint tissue in which clearly discernable rod-shaped glycoproteins were encountered in between collagen fibrils. Moreover, it appeared to stain larger deposits which might represent amyloid. Postlabeling with the immunogold method of the cuprolinic blue-stained tissue proved that cuprolinic blue positive fibrils represented AA-amyloid fibrils. Therefore, it was concluded that the GAGs which appeared to colocalize with the fibrillar microanatomy of amyloid, represent a structural part of the amyloid fibrils and that the avian amyloid fibrils may be considered as a pathological proteoglycan.
在国内的褐壳蛋鸡中，肝脏、脾脏等内脏器官以及关节的反应性淀粉样变性是一种常见病症。在包括鸡的AA型淀粉样蛋白在内的多种淀粉样蛋白类型中，除淀粉样纤维外，免疫组织化学检测或提取后还发现了蛋白聚糖和糖胺聚糖（GAGs）。本报告的目的是通过亚甲蓝染色和免疫金标记研究淀粉样纤维中GAGs的超微结构定位。兔抗鸡AA抗血清用于常规包埋和冷冻包埋的肝脏组织的免疫金标记，结果相似。因此，常规包块可用于进一步分析。对关节组织块进行亚甲蓝染色，在胶原纤维之间发现了清晰可辨的杆状糖蛋白。此外，它似乎能染出可能代表淀粉样蛋白的较大沉积物。对亚甲蓝染色组织进行免疫金法后标记，证明亚甲蓝阳性纤维代表AA型淀粉样纤维。因此，得出的结论是，似乎与淀粉样蛋白的纤维微观解剖结构共定位的GAGs是淀粉样纤维的结构组成部分，禽类淀粉样纤维可被视为一种病理性蛋白聚糖。
