Wang Kai, Mittler John E, Samudrala Ram
Department of Microbiology, University of Washington, Seattle, WA 98195, USA.
Science. 2006 May 12;312(5775):848; author reply 848. doi: 10.1126/science.1109904.
Bonhoeffer et al. (Reports, 26 November 2004, p. 1547) presented evidence for positive epistasis in a clinical data set of HIV-1 mutants and corresponding fitness values. We demonstrate that biases in the original and simulated data sets may lead to erroneous evidence for epistasis. More rigorous statistical tests must be used to account for such biases before one can infer epistasis.
邦赫费尔等人（《报告》，2004年11月26日，第1547页）在一组HIV-1突变体及相应适应度值的临床数据集中给出了正向上位效应的证据。我们证明，原始数据集和模拟数据集中的偏差可能导致关于上位效应的错误证据。在推断上位效应之前，必须使用更严格的统计检验来考虑此类偏差。
