Lucitti Jennifer L, Dickinson Mary E
Department of Molecular and Biophysics, Baylor College of Medicine, Houston, Texas 77071, USA.
Pediatr Res. 2006 Jul;60(1):1-5. doi: 10.1203/01.pdr.0000220318.49973.32. Epub 2006 May 11.
Fluorescence microscopy has become a principle methodology in the field of developmental biology. Recent technological advances have led to the design of high-speed and high-resolution confocal and multiphoton microscopes that enable researchers to obtain three- and four-dimensional information in living cells and whole embryos. Paralleling this progress, the development of stable and bright vital fluorescent probes has revolutionized the ability to track individual cells in vitro and in vivo and to visualize intercellular and subcellular molecular interactions in real time. Combining imaging modalities and labeling techniques that are increasingly unobtrusive to cell and whole animal function, our understanding of how proteins interact, tissues take form, and organs synchronize to create a functioning animal is reaching a whole new level.
荧光显微镜已成为发育生物学领域的一种主要方法。最近的技术进步带来了高速和高分辨率共聚焦及多光子显微镜的设计，使研究人员能够在活细胞和整个胚胎中获取三维和四维信息。与此进展并行的是，稳定且明亮的活体荧光探针的开发彻底改变了在体外和体内追踪单个细胞以及实时可视化细胞间和亚细胞分子相互作用的能力。结合对细胞和整个动物功能越来越不产生干扰的成像方式和标记技术，我们对蛋白质如何相互作用、组织如何形成以及器官如何协同运作以构建一个有功能的动物的理解正达到一个全新的水平。
