Garini Yuval, Vermolen Bart J, Young Ian T
Delft University of Technology, Lorentzweg 1, 2628 CJ, Delft, The Netherlands.
Curr Opin Biotechnol. 2005 Feb;16(1):3-12. doi: 10.1016/j.copbio.2005.01.003.
Improving the spatial resolution of optical microscopes is important for a vast number of applications in the life sciences. Optical microscopy allows intact samples and living cells to be studied in their natural environment, tasks that are not possible with other microscopy methods (e.g. electron microscopy). Major advances in the past two decades have significantly improved microscope resolution. By using interference and structured light methods microscope resolution has been improved to approximately 100 nm, and with non-linear methods a ten times improvement has been demonstrated to a current resolution limit of approximately 30 nm. These methods bring together old theoretical concepts such as interference with novel non-linear methods that improve spatial resolution beyond the limits that were previously assumed to be unreachable.
提高光学显微镜的空间分辨率对于生命科学中的大量应用而言至关重要。光学显微镜能够在自然环境中对完整样本和活细胞进行研究，而这些任务是其他显微镜方法（如电子显微镜）无法完成的。过去二十年的重大进展显著提高了显微镜的分辨率。通过使用干涉和结构光方法，显微镜分辨率已提高到约100纳米，并且采用非线性方法已证明分辨率提高了十倍，达到目前约30纳米的极限。这些方法将诸如干涉等古老的理论概念与新颖的非线性方法相结合，从而将空间分辨率提高到超出先前认为无法达到的极限。
