Hove Jay R
Department of Genome Science, Genome Research Institute, University of Cincinnati, OH 45237, USA.
Pediatr Res. 2006 Jul;60(1):6-13. doi: 10.1203/01.pdr.0000219584.22454.92. Epub 2006 May 11.
The relationship between developing biologic tissues and their dynamic fluid environments is intimate and complex. Increasing evidence supports the notion that these embryonic flow-structure interactions influence whether development will proceed normally or become pathogenic. Genetic, pharmacological, or surgical manipulations that alter the flow environment can thus profoundly influence morphologic and functional cardiovascular phenotypes. Functionally deficient phenotypes are particularly poorly described as there are few imaging tools with sufficient spatial and temporal resolution to quantify most intra-vital flows. The ability to visualize biofluids flow in vivo would be of great utility in functionally phenotyping model animal systems and for the elucidation of the mechanisms that underlie flow-related mechano-sensation and transduction in living organisms. This review summarizes the major methodological advances that have evolved for the quantitative characterization of intra-vital fluid dynamics with an emphasis on assessing cardiovascular flows in vertebrate model organisms.
发育中的生物组织与其动态流体环境之间的关系密切且复杂。越来越多的证据支持这样一种观点，即这些胚胎期的血流-结构相互作用会影响发育是否正常进行或是否会致病。因此，改变血流环境的基因、药理学或手术操作会深刻影响形态学和功能性心血管表型。由于几乎没有成像工具具有足够的空间和时间分辨率来量化大多数活体内部的血流，因此对功能缺陷表型的描述尤为不足。在功能上对模型动物系统进行表型分析以及阐明生物体内血流相关机械感受和转导的潜在机制方面，能够在体内可视化生物流体流动的能力将具有很大的实用价值。本综述总结了为定量表征活体内部流体动力学而发展的主要方法学进展，重点是评估脊椎动物模型生物中的心血管血流。
