Pearce B J, Wellington J E, Walker G J
Institute of Dental Research, United Dental Hospital, Surry Hills, New South Wales, Australia.
J Enzyme Inhib. 1991;5(2):99-110. doi: 10.3109/14756369109069063.
Cell-free filtrates of Streptococcus sobrinus, cultured at low growth rate in the chemostat, contain a dextranase inhibitor that can completely inhibit the activity of S. sobrinus endodextranase. The range of conditions under which inhibition occurs, and the situations in which enzyme activity can reappear, have been examined in continuous cultures of strain 6715-13WT and the dextranase-deficient mutant 6715-13-201. A purified preparation of the inhibitor was specific for S. sobrinus dextranase, having no action on dextranases from other oral streptococci. The percentage inhibition of S. sobrinus dextranase varied with the enzyme concentration, and the complete inhibition of low amounts of enzyme indicated a very tight bond between the inhibitor and the enzyme.
在恒化器中以低生长速率培养的远缘链球菌无细胞滤液含有一种葡聚糖酶抑制剂，该抑制剂可完全抑制远缘链球菌内切葡聚糖酶的活性。在菌株6715 - 13WT和葡聚糖酶缺陷型突变体6715 - 13 - 201的连续培养中，研究了发生抑制作用的条件范围以及酶活性可能重新出现的情况。该抑制剂的纯化制剂对远缘链球菌葡聚糖酶具有特异性，对其他口腔链球菌的葡聚糖酶没有作用。远缘链球菌葡聚糖酶的抑制百分比随酶浓度而变化，对少量酶的完全抑制表明抑制剂与酶之间的结合非常紧密。
