从离体兔主动脉条中释放内皮舒张因子（EDRF）的物质活性的定量分析。

Quantification of the potencies of EDRF-releasers from isolated rabbit aortic strips.

作者信息

Trybulec M, Dudek R, Radziszewski W, Swierkosz T, Zembowicz A

机构信息

Department of Pharmacology, Copernicus Academy of Medicine Cracow.

出版信息

Acta Physiol Pol. 1990;41(1-3):79-86.

PMID:1669477

Abstract

We have compared several known releasers of endothelium-derived relaxing factor (EDRF)(13) in respect to their potencies to generate EDRF by endothelium of rabbit aortic strips (RbA) superfused with Krebs' buffer. The vasorelaxation by EDRF which is equivalent to 10 pmoles of GTN was evoked by 0.7 pmoles of substance P(SP), 50 pmoles of acetylcholine (Ach), 521 pmoles of calcium ionophore A 23187, 2720 pmoles of ADP. Threshold potencies of these agonists are inversely proportional to the maximum amount of EDRF released. Phospholipase C (PLC) from Clostridium perfringens at a dose of 0.1 U caused the relaxation of a similar magnitude. Phospholipase A2 (1 U), thrombin (1 U), bradykinin (30 nmoles) and serotonin (10 pmoles) did not release EDRF. It is concluded that endothelial cells of RbA differ from endothelial cells of other species in their susceptibility to release EDRF in response to various agonists.

摘要

我们比较了几种已知的内皮源性舒张因子（EDRF）释放剂，评估它们在与Krebs缓冲液灌注的兔主动脉条（RbA）内皮中产生EDRF的能力。相当于10皮摩尔硝酸甘油（GTN）的EDRF引起的血管舒张，分别由0.7皮摩尔P物质（SP）、50皮摩尔乙酰胆碱（Ach）、521皮摩尔钙离子载体A23187、2720皮摩尔ADP诱发。这些激动剂的阈效价与释放的EDRF最大量成反比。来自产气荚膜梭菌的磷脂酶C（PLC），剂量为0.1单位，引起了相似程度的舒张。磷脂酶A2（1单位）、凝血酶（1单位）、缓激肽（30纳摩尔）和5-羟色胺（10皮摩尔）不释放EDRF。结论是，RbA的内皮细胞在对各种激动剂释放EDRF的敏感性方面与其他物种的内皮细胞不同。