Palmer R M, Ferrige A G, Moncada S
Nature. 1987;327(6122):524-6. doi: 10.1038/327524a0.
Endothelium-derived relaxing factor (EDRF) is a labile humoral agent which mediates the action of some vasodilators. Nitrovasodilators, which may act by releasing nitric oxide (NO), mimic the effect of EDRF and it has recently been suggested by Furchgott that EDRF may be NO. We have examined this suggestion by studying the release of EDRF and NO from endothelial cells in culture. No was determined as the chemiluminescent product of its reaction with ozone. The biological activity of EDRF and of NO was measured by bioassay. The relaxation of the bioassay tissues induced by EDRF was indistinguishable from that induced by NO. Both substances were equally unstable. Bradykinin caused concentration-dependent release of NO from the cells in amounts sufficient to account for the biological activity of EDRF. The relaxations induced by EDRF and NO were inhibited by haemoglobin and enhanced by superoxide dismutase to a similar degree. Thus NO released from endothelial cells is indistinguishable from EDRF in terms of biological activity, stability, and susceptibility to an inhibitor and to a potentiator. We suggest that EDRF and NO are identical.
内皮源性舒张因子（EDRF）是一种不稳定的体液因子，它介导某些血管舒张剂的作用。硝基血管舒张剂可能通过释放一氧化氮（NO）起作用，模拟EDRF的效应，最近弗奇戈特提出EDRF可能就是NO。我们通过研究培养的内皮细胞释放EDRF和NO来检验这一假说。NO被测定为其与臭氧反应的化学发光产物。EDRF和NO的生物活性通过生物测定法进行测量。EDRF诱导的生物测定组织的舒张与NO诱导的舒张没有区别。两种物质同样不稳定。缓激肽引起细胞以足以解释EDRF生物活性的量浓度依赖性释放NO。EDRF和NO诱导的舒张被血红蛋白抑制，并被超氧化物歧化酶增强到相似程度。因此，就生物活性、稳定性以及对抑制剂和增强剂的敏感性而言，内皮细胞释放的NO与EDRF没有区别。我们认为EDRF和NO是相同的。
