Hu Jia, Lan Xiaoli, Zhang Yongxue, Gao Zairong, Wu Jun
Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(6):712-4. doi: 10.1007/BF02896179.
To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain, which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG3. (1) Preparation of the 99mTc-YIGSR probe: with S-Acetly-NH3-MAG3 as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EAC's uptake of 99mTc-YIGSR and 99mTc-MIBI was observed at 37 degrees C and 22 degrees C, respectively. (1) The labeling efficiencies of 99mTc-YIGSR and 99mTc-MIBI were (62 +/- 3)% and (96 +/- 2)%, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 degrees C, the EAC'S uptake of 99mTc-YIGSR and 99mTc-MIBI reached the peak of (43.16 +/- 2.4) % and (24.4 +/- 1.8) % at 60 min, respectively; and at 22 degrees C, the highest uptake was (26.5 +/- 2.1) % and (9.47 +/- 1.9) % at 60 min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.
为研究一种新型肿瘤示踪剂99mTc-YIGSR，其由层粘连蛋白β链的五肽结构（YIGSR）衍生而来，能特异性结合肿瘤的层粘连蛋白受体，并以MAG3进行放射性标记。（1）99mTc-YIGSR探针的制备：以S-乙酰基-NH3-MAG3为螯合剂，用适当的还原剂将YIGSR用99mTc标记；（2）细胞培养及活力测定：艾氏腹水癌（EAC）细胞在添加小牛血清的RPMI 1640培养基中培养；采用台盼蓝拒染法计算细胞活力；（3）细胞动力学研究：分别在37℃和22℃观察EAC细胞对99mTc-YIGSR和99mTc-MIBI的摄取。（1）99mTc-YIGSR和99mTc-MIBI的标记率分别为（62±3）%和（96±2）%；（2）细胞活力随孵育时间下降；（3）在37℃时，EAC细胞对99mTc-YIGSR和99mTc-MIBI的摄取分别在60分钟时达到峰值（43.16±2.4）%和（24.4±1.8）%；在22℃时，60分钟时最高摄取率分别为（26.5±2.1）%和（9.47±1.9）%。体外研究表明，99mTc-YIGSR在细胞摄取方面优于99mTc-MIBI，在肿瘤显像方面具有潜在价值。
