Soukup Tomás, Mokrý Jaroslav, Karbanová Jana, Pytlík Robert, Suchomel Petr, Kucerová Lenka
Department of Histology and Embryology, Charles University in Prague, Faculty of Medicine, Hradec Králové, Czech Republic.
Acta Medica (Hradec Kralove). 2006;49(1):27-33.
Mesenchymal Stem Cells (MSCs) are rare elements living in various organs (e.g., bone marrow), able to differentiate into specialized tissues, such as bone, cartilage, tendon, and myocardium. Since the first description of MSCs by Fridenshtein, several investigators have shown that these cells can also differentiate into chondrocytes, adipocytes, and, at least, in rodents into skeletal myoblasts. Later on, more primitive progenitor cells were characterized, able to give rise not only to limb-bud mesoderm, but also to cells of visceral mesoderm. Those cells were named mesodermal progenitor cells (MPCs). The aim of our study was to characterize and compare the biological properties and spontaneous differentiation potential of two different cell types (MSCs and MPCs) isolated from the human vertebral body bone marrow. The results of our experiments proved that the MPCs can be expanded beyond Hayflick's limit and differed from MSCs in morphology, biological and phenotypic characteristics. Because of their high proliferative and differentiation potential, MPCs can become more attractive source of adult stem cells for therapeutic purposes.
间充质干细胞（MSCs）是存在于各种器官（如骨髓）中的稀有细胞，能够分化为特殊组织，如骨骼、软骨、肌腱和心肌。自弗里登施泰因首次描述MSCs以来，一些研究人员表明，这些细胞还可分化为软骨细胞、脂肪细胞，至少在啮齿动物中可分化为骨骼肌成肌细胞。后来，更多原始祖细胞被鉴定出来，它们不仅能产生肢芽中胚层，还能产生内脏中胚层细胞。这些细胞被命名为中胚层祖细胞（MPCs）。我们研究的目的是对从人椎体骨髓中分离出的两种不同细胞类型（MSCs和MPCs）的生物学特性和自发分化潜能进行鉴定和比较。我们的实验结果证明，MPCs可以扩增超过海弗利克极限，并且在形态、生物学和表型特征方面与MSCs不同。由于其高增殖和分化潜能，MPCs可能成为用于治疗目的的更具吸引力的成体干细胞来源。
