Institute of Sports Medicine, Peking University Third Hospital, Beijing, PR China.
Tissue Eng Part A. 2012 Sep;18(17-18):1793-803. doi: 10.1089/ten.TEA.2011.0530. Epub 2012 Jul 30.
Mesenchymal stem cells (MSCs) from adult exhibit self-renewal and multilineage differentiation capacities, making the MSCs promising candidates for cell therapy and tissue engineering. Although bone marrow (BM) is the most universal source of MSCs, other tissues may also contain MSCs. Peripheral blood (PB), in particular, arises as the most attractive source of MSCs due to easy accessibility and noninvasive procedure. However, it is not certain that PB-MSCs have the equal biological characteristics to those of BM-MSCs. The purpose of this study was to compare the biological characteristics between BM-MSCs and PB-MSCs. We adopted granulocyte colony-stimulating factor combined with CXCR4 antagonist AMD3100 to stimulate MSCs to release into blood circulation of the rats. PB-MSCs were obtained from mobilized PB and expanded in long-term culture. BM-MSCs were isolated from the femur and tibia medullary canal of the same rats by density gradient centrifugation. After cell expansion in vitro, cell surface markers and multipotentiality analysis were performed to identify MSCs. Apoptosis resistance to H(2)O(2)-induced apoptosis, proliferation kinetics, cellular senescence, and karyotype analysis were measured to compare the biological characteristics of PB-MSCs and BM-MSCs. PB-MSCs with the typical adherent fibroblast-like morphology were similar to that of BM-MSCs. Both PB-MSCs and BM-MSCs were positive for CD44 and CD90, and negative for CD34 and CD45. They both exhibited trilineage differentiation potential and expressed lineage-specific genes. Although the BM-MSCs showed stronger osteogenic and adipogenic differentiation, PB-MSCs displayed a more chondrogenic capacity. Further, BM-MSCs have greater proliferation ability. Apoptosis resistance and cellular senescence were similar in MSCs derived from both sources. The results of our study demonstrate that PB-MSCs have similar biological characteristics to those of BM-MSCs despite certain minor differences, suggesting PB as a possible alternative source for MSCs.
间充质干细胞（MSCs）来源于成体，具有自我更新和多向分化能力，使其成为细胞治疗和组织工程的有前途的候选者。尽管骨髓（BM）是最普遍的 MSC 来源，但其他组织也可能含有 MSC。特别是外周血（PB）由于易于获得和非侵入性程序，成为最有吸引力的 MSC 来源。然而，PB-MSCs 是否具有与 BM-MSCs 相等的生物学特性尚不确定。本研究的目的是比较 BM-MSCs 和 PB-MSCs 之间的生物学特性。我们采用粒细胞集落刺激因子联合 CXCR4 拮抗剂 AMD3100 刺激 MSC 释放到大鼠的血液循环中。从动员的 PB 中获得 PB-MSCs，并在长期培养中进行扩增。通过密度梯度离心从同一大鼠的股骨和胫骨骨髓腔中分离 BM-MSCs。体外细胞扩增后，进行细胞表面标志物和多能性分析以鉴定 MSC。测量对 H（2）O（2）诱导的细胞凋亡的抗凋亡能力、增殖动力学、细胞衰老和核型分析，以比较 PB-MSCs 和 BM-MSCs 的生物学特性。具有典型贴壁成纤维样形态的 PB-MSCs 与 BM-MSCs 相似。PB-MSCs 和 BM-MSCs 均对 CD44 和 CD90 呈阳性，对 CD34 和 CD45 呈阴性。它们均表现出三系分化潜能，并表达谱系特异性基因。尽管 BM-MSCs 表现出更强的成骨和脂肪分化能力，但 PB-MSCs 表现出更强的软骨形成能力。此外，BM-MSCs 具有更强的增殖能力。MSC 来源的细胞凋亡抵抗和细胞衰老相似。我们的研究结果表明，尽管存在某些细微差异，PB-MSCs 具有与 BM-MSCs 相似的生物学特性，提示 PB 可能是 MSC 的一种潜在替代来源。
