Lukácsi Gyöngyi, Takó M, Nyilasi Ildikó
Department of Microbiology, Faculty of Sciences, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary.
Acta Microbiol Immunol Hung. 2006 Mar;53(1):95-104. doi: 10.1556/AMicr.53.2006.1.7.
The separation of chromosome-size DNA molecules by pulsed-field gel electrophoresis (PFGE) has become a well-established technique in recent years. Although it has very wide-ranging applications, it made a real breakthrough for fungal genome analysis. Because of the small size of fungal chromosomes, their investigation was not possible earlier. Different PFGE approaches allowed the separation of DNA molecules larger than 10 megabase pairs in size, and electrophoretic karyotypes for numerous previously genetically uncharacterized fungal species could be established. This review discusses the applicability of these electrophoretic karyotypes for the investigation of genome structure, for strain identification and for species delimitation.
近年来，通过脉冲场凝胶电泳（PFGE）分离染色体大小的DNA分子已成为一项成熟的技术。尽管它有非常广泛的应用，但它在真菌基因组分析方面取得了真正的突破。由于真菌染色体尺寸小，早期无法对其进行研究。不同的PFGE方法能够分离大小超过10兆碱基对的DNA分子，并且可以建立许多先前遗传特征未知的真菌物种的电泳核型。本文综述讨论了这些电泳核型在基因组结构研究、菌株鉴定和物种界定方面的适用性。
