Mehrabi Rahim, Taga Masatoki, Aghaee Mostafa, de Wit Pierre J G M, Kema Gert H J
Seed and Plant Improvement Institute, Karaj, Iran.
Methods Mol Biol. 2012;835:591-602. doi: 10.1007/978-1-61779-501-5_37.
Pulsed field gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes.
脉冲场凝胶电泳能够分离长达数兆碱基的真菌染色体，是真菌核型分析的一项有价值的工具。芽管破裂法是一种分离任何大小的真菌染色体以进行染色体数目测定以及原位杂交的技术。在这里，我们提供了这两种互补方法的详细方案，它们在真菌生物学中有许多应用，包括染色体大小和染色体数目多态性，以及基因在染色体上的原位定位。
