Costa Af Ali U M, Abreu Ana L, Gomes Claudia M C, Saldanha Ana C R, Barral A, Costa Jackson M L, Corbett Carlos E P
Nucleus of Tropical Pathology and Social Medicine, Federal University of Maranhão, São Luís, Maranhão, Brazil.
Acta Trop. 2006 May;98(2):125-9. doi: 10.1016/j.actatropica.2006.02.007. Epub 2006 May 11.
Experimental animal models have been used for the study of the physiopathogenesis of leishmaniasis, on some occasions with success, while in other situations such as bone alterations that accompany tegumentary leishmaniasis, especially in diffuse cutaneous form (DCL), the mechanisms are still unknown. In the present study, we determined these alterations in an animal model susceptible to Leishmania (L) amazonensis. Amastigotes of L. (L) amazonensis isolated from patients with diffuse cutaneous leishmaniasis (DCL) were inoculated into the hind paws of eight BALB/c mice, macroscopic and histopathological aspects were analyzed. After 90 and 120 days of evolution, histopathological analysis demonstrated a mononuclear cell infiltrate rich in plasma cells and intense parasitism of intra- and extra-medullary macrophages, with areas of bone necrosis and discrete involvement of cartilaginous tissue. The results show that the inflammatory process developed during L. (L) amazonensis infection might cause bone tissue destruction and secondarily affect the joints.
实验动物模型已被用于利什曼病生理病理发生机制的研究，某些情况下取得了成功，而在其他情形中，如皮肤利什曼病伴随的骨骼改变，尤其是弥漫性皮肤型（DCL），其机制仍不清楚。在本研究中，我们在一种对亚马逊利什曼原虫（L）易感的动物模型中确定了这些改变。从弥漫性皮肤利什曼病（DCL）患者分离出的亚马逊利什曼原虫（L）无鞭毛体接种到8只BALB/c小鼠的后爪，分析宏观和组织病理学方面的情况。在病程90天和120天后，组织病理学分析显示有富含浆细胞的单核细胞浸润以及髓内和髓外巨噬细胞的强烈寄生现象，伴有骨坏死区域和软骨组织的轻微受累。结果表明，亚马逊利什曼原虫（L）感染期间发生的炎症过程可能导致骨组织破坏，继而影响关节。
