Zana Marianna, Szécsényi Anita, Czibula Agnes, Bjelik Annamária, Juhász Anna, Rimanóczy Agnes, Szabó Krisztina, Vetró Agnes, Szucs Péter, Várkonyi Agnes, Pákáski Magdolna, Boda Krisztina, Raskó István, Janka Zoltán, Kálmán János
Department of Psychiatry, Alzheimer's Disease Research Center, Faculty of Medicine, Albert Szent-Györgyi Center for Medical and Pharmaceutical Sciences, University of Szeged, 6 Semmelweis St., Szeged, H-6725, Hungary.
Biochem Biophys Res Commun. 2006 Jun 30;345(2):726-33. doi: 10.1016/j.bbrc.2006.04.167. Epub 2006 May 5.
The aim of the present study was to investigate the oxidative status of lymphocytes from children (n=7) and adults (n=18) with Down's syndrome (DS). The basal oxidative condition, the vulnerability to in vitro hydrogen peroxide exposure, and the repair capacity were measured by means of the damage-specific alkaline comet assay. Significantly and age-independently elevated numbers of single strand breaks and oxidized bases (pyrimidines and purines) were found in the nuclear DNA of the lymphocytes in the DS group in the basal condition. These results may support the role of an increased level of endogenous oxidative stress in DS and are similar to those previously demonstrated in Alzheimer's disease. In the in vitro oxidative stress-induced state, a markedly higher extent of DNA damage was observed in DS children as compared with age- and gender-matched healthy controls, suggesting that young trisomic lymphocytes are more sensitive to oxidative stress than normal ones. However, the repair ability itself was not found to be deteriorated in either DS children or DS adults.
本研究的目的是调查患有唐氏综合征（DS）的儿童（n = 7）和成人（n = 18）淋巴细胞的氧化状态。通过损伤特异性碱性彗星试验测量基础氧化状态、体外过氧化氢暴露的易感性和修复能力。在基础状态下，DS组淋巴细胞的核DNA中发现单链断裂和氧化碱基（嘧啶和嘌呤）的数量显著增加，且与年龄无关。这些结果可能支持内源性氧化应激水平升高在DS中的作用，并且与先前在阿尔茨海默病中证明的结果相似。在体外氧化应激诱导状态下，与年龄和性别匹配的健康对照相比，DS儿童中观察到明显更高程度的DNA损伤，这表明年轻的三体淋巴细胞比正常淋巴细胞对氧化应激更敏感。然而，未发现DS儿童或DS成人的修复能力本身有所下降。
