脂肪组织来源的成体干细胞在裸鼠体内的成脂分化

Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse.

作者信息

Choi Yu Suk, Cha Sang Myun, Lee Young Yeol, Kwon Seok Woon, Park Chan Jeoung, Kim MiJung

机构信息

Asan Institute for life sciences, University of Ulsan, and Sein Aesthetic Plastic Surgery Clinic, Seoul 138-736, Republic of Korea.

出版信息

Biochem Biophys Res Commun. 2006 Jun 30;345(2):631-7. doi: 10.1016/j.bbrc.2006.04.128. Epub 2006 May 2.

DOI:10.1016/j.bbrc.2006.04.128

PMID:16696950

Abstract

We evaluated the use of a combination of adipose tissue derived adult stem cells (ADSCs) obtained from liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for adipose tissue engineering. Adipogenesis was examined in nude mice injected subcutaneously with ADSCs (group I), PLGA spheres (group II), or ADSCs attached PLGA spheres (group III) cultured in adipogenic medium for 7 days. After 4 and 8 weeks, newly formed adipose tissue was observed in groups II and III but not in group I. Oil red O staining of newly formed tissue showed that there was substantially more tissue regeneration and adipogenic differentiation in group III than in group II. RT-PCR confirmed that, after 8 weeks, the PLGA-attached ADSCs had fully differentiated into adipocytes. This study provides significant evidence that ADSCs and PLGA spheres can be used in a clinical setting to generate adipose tissue as a noninvasive soft tissue filler.

摘要

我们评估了抽脂获得的脂肪组织来源的成体干细胞（ADSCs）与可注射聚乳酸-乙醇酸共聚物（PLGA）球体联合用于脂肪组织工程的情况。在皮下注射了ADSCs（第一组）、PLGA球体（第二组）或附着有ADSCs的PLGA球体（第三组）并在成脂培养基中培养7天的裸鼠体内检测脂肪生成情况。4周和8周后，在第二组和第三组中观察到新形成的脂肪组织，而第一组中未观察到。对新形成组织的油红O染色显示，第三组中的组织再生和脂肪生成分化明显多于第二组。逆转录-聚合酶链反应（RT-PCR）证实，8周后，附着PLGA的ADSCs已完全分化为脂肪细胞。本研究提供了重要证据，表明ADSCs和PLGA球体可在临床环境中用作无创软组织填充剂来生成脂肪组织。