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人脂肪来源干细胞促进移植到裸鼠体内的胶原基支架血管化。

Human adipose-derived stem cells promote vascularization of collagen-based scaffolds transplanted into nude mice.

作者信息

Cherubino Mario, Valdatta Luigi, Balzaretti Riccardo, Pellegatta Igor, Rossi Federica, Protasoni Marina, Tedeschi Alessandra, Accolla Roberto S, Bernardini Giovanni, Gornati Rosalba

机构信息

Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.

Dipartimento di Scienze Chirurgiche e Morfologiche, Università degli Studi dell'Insubria, Via Guicciardini 9, 21100 Varese, Italy.

出版信息

Regen Med. 2016 Apr;11(3):261-71. doi: 10.2217/rme-2015-0010. Epub 2016 Mar 11.

DOI:10.2217/rme-2015-0010
PMID:26965659
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4976995/
Abstract

AIM

After in vivo implantation of cell-loaded devices, only the cells close to the capillaries can obtain nutrients to maintain their functions. It is known that factors secreted by stem cells, rather than stem cells themselves, are fundamental to guarantee new vascularization in the area of implant.

MATERIALS & METHODS: To investigate this possibility, we have grafted mice with Bilayer and Flowable Integra(®) scaffolds, loaded or not with human adipose-derived stem cells.

RESULTS

Our results support the therapeutic potential of human adipose-derived stem cells to induce new vascular networks of engineered organs and tissues.

CONCLUSION

This finding suggests that our approach can help to form new vascular networks that allow sufficient vascularization of engineered organs and tissues in cases of difficult wound healing due to ischemic conditions.

摘要

目的

在体内植入载有细胞的装置后，只有靠近毛细血管的细胞才能获取营养以维持其功能。众所周知，干细胞分泌的因子而非干细胞本身是保证植入区域新生血管形成的关键因素。

材料与方法

为探究这种可能性，我们将双层和可流动的Integra(®)支架移植到小鼠体内，这些支架加载或未加载人脂肪干细胞。

结果

我们的结果支持人脂肪干细胞在诱导工程化器官和组织形成新血管网络方面的治疗潜力。

结论

这一发现表明，我们的方法有助于形成新的血管网络，在因缺血状况导致伤口愈合困难的情况下，使工程化器官和组织获得充足的血管化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/93e9a7a2f7c5/rme-11-261-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/844ab722ac1a/rme-11-261-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/07f141a6f04f/rme-11-261-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/b66fe0c28789/rme-11-261-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/561184810c75/rme-11-261-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/4149adccebeb/rme-11-261-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/93e9a7a2f7c5/rme-11-261-g6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/844ab722ac1a/rme-11-261-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/07f141a6f04f/rme-11-261-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/b66fe0c28789/rme-11-261-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/561184810c75/rme-11-261-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/4149adccebeb/rme-11-261-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b30/4976995/93e9a7a2f7c5/rme-11-261-g6.jpg

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