Hoshiba Takashi, Cho Chong Su, Murakawa Akiko, Okahata Yoshio, Akaike Toshihiro
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Biomaterials. 2006 Sep;27(26):4519-28. doi: 10.1016/j.biomaterials.2006.04.014. Epub 2006 May 11.
It is well known that natural extracellular matrix (ECM) molecules are deposited on the surface of biomaterials during culture of cells and affect cellular behaviors. However, it has not been fully understood what kinds of ECM molecules are deposited on the surface of biomaterials although the cellular behaviors were affected by deposited ECM. In this study, to investigate the effect of deposited natural ECM on behaviors of hepatocytes cultured on biomaterials such as poly (N-p-vinylbenzyl-4-O-beta-D-galactopyranosyl-D-gluconamide) (PVLA) as a hepatocyte-specific matrix and poly (L-lysine) (PLL) as a non-specific one during the culture of hepatocytes in vitro, we investigated expression pattern of ECM genes and adsorption of ECM molecules onto PVLA- and PLL-coated surfaces. It was found that the expression levels of type I collagen and fibronectin genes in the hepatocytes cultured on PVLA-coated surface were different from them in the hepatocytes cultured on PLL-coated one. Also, the results showed that laminin was dominantly deposited on PVLA-coated surface whereas fibronectin was dominantly deposited on PLL-coated one. Hepatocytes maintained liver-specific functions on PVLA- and laminin-coated surfaces. It is thought that deposited laminin during the culture of hepatocytes affects the liver-specific functions of hepatocytes cultured on PVLA-coated surface.
众所周知,在细胞培养过程中,天然细胞外基质(ECM)分子会沉积在生物材料表面并影响细胞行为。然而,尽管细胞行为受到沉积的ECM的影响,但对于沉积在生物材料表面的ECM分子种类尚未完全了解。在本研究中,为了研究在体外培养肝细胞期间,沉积的天然ECM对在诸如作为肝细胞特异性基质的聚(N-对乙烯基苄基-4-O-β-D-吡喃半乳糖基-D-葡糖酰胺)(PVLA)和作为非特异性基质的聚(L-赖氨酸)(PLL)等生物材料上培养的肝细胞行为的影响,我们研究了ECM基因的表达模式以及ECM分子在PVLA和PLL包被表面的吸附情况。结果发现,在PVLA包被表面培养的肝细胞中I型胶原蛋白和纤连蛋白基因的表达水平与在PLL包被表面培养的肝细胞中的不同。此外,结果表明,层粘连蛋白主要沉积在PVLA包被的表面,而纤连蛋白主要沉积在PLL包被的表面。肝细胞在PVLA和层粘连蛋白包被的表面上维持肝脏特异性功能。据认为,在肝细胞培养过程中沉积的层粘连蛋白会影响在PVLA包被表面培养的肝细胞的肝脏特异性功能。