Braak H, Braak E
Department of Morphology, Johann Wolfgang Goethe University, Frankfurt, Federal Republic of Germany.
Brain Pathol. 1991 Apr;1(3):213-6. doi: 10.1111/j.1750-3639.1991.tb00661.x.
Selective and sensitive silver staining of extracellular amyloid deposits and intraneuronal neurofibrillary changes can be applied to 50-150 microns thick polyethylene glycol sections and/or 5-15 microns thick paraffin sections. The silver techniques take advantage of physical development of the nucleation sites thus permitting tight control of the entire procedure. Both techniques can be applied to routinely fixed autopsy material. They do not require particular skills and considerably facilitate processing of large numbers of sections through entire hemispheres of the human brain.
细胞外淀粉样沉积物和神经元内神经原纤维变化的选择性和灵敏银染色可应用于50 - 150微米厚的聚乙二醇切片和/或5 - 15微米厚的石蜡切片。银染技术利用成核位点的物理显影，从而允许对整个过程进行严格控制。这两种技术都可应用于常规固定的尸检材料。它们不需要特殊技能，并且极大地便于处理通过整个人脑半球的大量切片。
