Reusche E
Institute of Pathology, Medical University Lübeck, FRG.
Pathol Res Pract. 1991 Dec;187(8):1045-9. doi: 10.1016/S0344-0338(11)81084-8.
A one-step silver staining method using a protective colloidal developer of gelatin and formic acid was originally established for demonstration of argyrophilic nucleolus organizer regions by controlled reduction of silver. We describe here a new application of this silver technique that can easily be performed to demonstrate senile plaques (SP) and neurofibrillary tangles (NFT) on paraffin sections. Preliminary results in ten cases of senile dementia of Alzheimer type and five cases of amyloid congophilic angiopathy showed a reliable demonstration of amyloid and neuritic type SP as well as NFT in all 15 cases. In addition, deposits of perivascular amyloid and areas of fibrillar amyloid, the diffuse type of senile plaques, were revealed. The success seems to depend particularly on the low concentration of formic acid in the developer, which might be the responsible agent for the careful revealing of buried argyrophilic structures in SP and NFT. The staining features were quite similar to those with anti-beta and anti-tau immunostaining. This result suggests a high specificity of this method for extracellular and intraneuronal cerebral amyloid. The detailed staining of delicate morphological structures points to a high sensitivity for cerebral amyloids, senile plaques and neurofibrillary tangles, respectively, by this simple and inexpensive method.
一种使用明胶和甲酸作为保护性胶体显影剂的一步银染法最初是为通过控制银的还原以显示嗜银核仁组织区而建立的。我们在此描述这种银染技术的一种新应用，该应用可轻松用于在石蜡切片上显示老年斑（SP）和神经原纤维缠结（NFT）。对10例阿尔茨海默型老年痴呆症患者和5例嗜刚果红血管病患者的初步结果显示，在所有15例病例中均能可靠地显示淀粉样蛋白和神经炎型老年斑以及神经原纤维缠结。此外，还发现了血管周围淀粉样蛋白沉积和纤维状淀粉样蛋白区域，即弥漫型老年斑。成功似乎尤其取决于显影剂中甲酸的低浓度，这可能是小心显示老年斑和神经原纤维缠结中埋藏的嗜银结构的关键因素。染色特征与抗β和抗tau免疫染色的特征非常相似。这一结果表明该方法对细胞外和细胞内脑淀粉样蛋白具有高度特异性。通过这种简单且廉价的方法，对精细形态结构的详细染色分别显示出对脑淀粉样蛋白、老年斑和神经原纤维缠结具有高敏感性。
